上皮细胞黏附分子和 TROP2 在非小细胞肺癌中的表达意义。
Significance of EpCAM and TROP2 expression in non-small cell lung cancer.
机构信息
Department of Pathology, School of Medicine, Pusan National University, Beomeo-ri, Mulgeum-eup, Yangsan 626-770, South Korea.
出版信息
World J Surg Oncol. 2012 Apr 6;10:53. doi: 10.1186/1477-7819-10-53.
BACKGROUND
The tumor-associated calcium signal transducer (TACSTD) genes, originally designated epithelial cell adhesion molecule (EpCAM) and TROP2, represent true oncogenes. Little is known about EpCAM and TROP2 gene expression in non-small cell lung carcinoma (NSCLC). This study evaluated EpCAM and TROP2 protein expression and clinicopathologic significance in cases of NSCLC.
METHODS
Tissue microarray blocks acquired from 164 cases of NSCLC, including 100 cases of adenocarcinoma (AdC) and 64 of squamous cell carcinoma (SCC), were examined by immunohistochemical staining for EpCAM, and TROP2. The results were correlated with clinicopathologic data.
RESULTS
EpCAM and TROP2 were significantly overexpressed in SCC than in AdC (P < 0.01). In AdC, EpCAM overexpression was closely related to sex, histologic grade, pathologic T stage, pathologic N stage, and TNM stage, and TROP2 overexpression was only related to histologic grade (P < 0.05, respectively). In SCC, correlations were evident between EpCAM overexpression and TNM stage (P = 0.01), and between TROP2 overexpression and pathologic T stage (P = 0.02). EpCAM overexpression showed no significance with overall survival in AdC and SCC patients. However, TROP2 overexpression in AdC had a positive influence on overall survival (P = 0.02) and disease-free survival (P = 0.03). In particular, AdC patients with stage II or III showed better overall survival (P = 0.05) and disease-free survival (P = 0.04).
CONCLUSIONS
While EpCAM and TROP2 show weak and non-complete membranous staining in normal bronchial epithelium and pneumocyte, their complete membranous expression in carcinoma suggests their role in carcinogenesis. EpCAM and TROP2 were more frequently overexpressed in SCC. EpCAM overexpression had no prognostic value in this study, but TROP2 overexpression showed better survival in AdC patients and might be a better prognostic marker in advanced stage AdC.
背景
肿瘤相关钙信号转导蛋白（TACSTD）基因，最初被命名为上皮细胞黏附分子（EpCAM）和 TROP2，代表真正的癌基因。目前对于非小细胞肺癌（NSCLC）中 EpCAM 和 TROP2 基因的表达知之甚少。本研究评估了 EpCAM 和 TROP2 蛋白在 NSCLC 病例中的表达及其临床病理意义。
方法
通过免疫组织化学染色检测 164 例 NSCLC 组织微阵列块中 EpCAM 和 TROP2 的表达，包括 100 例腺癌（AdC）和 64 例鳞状细胞癌（SCC）。结果与临床病理数据相关。
结果
与 AdC 相比，SCC 中 EpCAM 和 TROP2 的表达显著升高（P < 0.01）。在 AdC 中，EpCAM 的过表达与性别、组织学分级、病理 T 分期、病理 N 分期和 TNM 分期密切相关，而 TROP2 的过表达仅与组织学分级相关（P < 0.05，分别）。在 SCC 中，EpCAM 过表达与 TNM 分期之间存在显著相关性（P = 0.01），TROP2 过表达与病理 T 分期之间存在显著相关性（P = 0.02）。在 AdC 和 SCC 患者中，EpCAM 过表达与总生存期无关，但 TROP2 过表达与总生存期（P = 0.02）和无病生存期（P = 0.03）呈正相关。特别是在 II 期或 III 期的 AdC 患者中，总生存期（P = 0.05）和无病生存期（P = 0.04）更好。
结论
虽然 EpCAM 和 TROP2 在正常支气管上皮和肺细胞中显示弱的、不完全的膜染色，但它们在癌组织中的完全膜表达表明它们在致癌作用中的作用。EpCAM 和 TROP2 在 SCC 中更常过度表达。在本研究中，EpCAM 过表达没有预后价值，但 TROP2 过表达在 AdC 患者中显示出更好的生存结果，并且可能是晚期 AdC 的更好预后标志物。
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