Veterinary Research Institute, National Agricultural Research Foundation, NAGREF Campus, PO Box 60272, 57001 Thermi, Greece.
Vet Parasitol. 2012 Aug 13;188(1-2):1-9. doi: 10.1016/j.vetpar.2012.03.014. Epub 2012 Mar 16.
The effect of sainfoin (Onobrychis viciifolia) against ovine coccidia was evaluated in vivo and in vitro. In 3 in vivo trials weaned lambs were allocated into two treatment groups receiving diets with either lucerne (Medicago sativa) or sainfoin. During the trials, which lasted for 7 (trial 1) or 8 weeks (trials 2 and 3), oocysts per gram of faeces (OPGs), faecal scores and weight gain were recorded. In two of the experiments (trials 1 and 3) a reduction in the mean oocyst excretion rates was observed, starting three to four weeks after sainfoin hay feeding. This reduction ranged between 21.3% (trial 1) and 61.7% (trial 3) compared to the control values. As a result, a decrease in the total number of oocysts excreted (expressed as the mean area under the curve of the OPG) was observed from week 4 to the end of the two trials, respectively (trial 1: 42.6% reduction, p=0.05; trial 3: 52.4% reduction, p=0.06). The results did not show any significant diet effect on lamb growth rates and faecal scores. In the in vitro experiments the effect of 39 sainfoin extracts were tested in an oocyst sporulation inhibition assay. The Eimeria oocysts sporulation inhibition throughout the experiments did not exceed 10.7%, showing that extracts of this forages do not have a significant inhibitory effect on Eimeria oocyst sporulation. This was an initial attempt to investigate a possible anticoccidial effect of sainfoin and further studies are needed in order to better understand its mode of action against Eimeria.
苦马豆（Onobrychis viciifolia）对绵羊球虫的作用在体内和体外进行了评估。在 3 项体内试验中，断奶羔羊被分配到接受含紫花苜蓿（Medicago sativa）或苦马豆的日粮的两组治疗中。在持续 7 周（试验 1）或 8 周（试验 2 和 3）的试验期间，记录每克粪便中的卵囊数（OPG）、粪便评分和体重增加。在其中两项试验（试验 1 和 3）中，在苦马豆干草喂养后三到四周开始观察到平均卵囊排泄率降低。与对照值相比，这种降低幅度在 21.3%（试验 1）至 61.7%（试验 3）之间。因此，从第 4 周开始，在这两项试验的其余时间观察到排出的总卵囊数（表示为 OPG 的平均曲线下面积）减少（试验 1：减少 42.6%，p=0.05；试验 3：减少 52.4%，p=0.06）。结果显示，日粮对羔羊生长速度和粪便评分没有显著影响。在体外试验中，用 39 种苦马豆提取物在卵囊孢子化抑制试验中进行了测试。整个试验过程中，Eimeria 卵囊孢子化抑制率不超过 10.7%，表明这种饲料的提取物对 Eimeria 卵囊孢子化没有显著的抑制作用。这是首次尝试研究苦马豆可能的抗球虫作用，需要进一步的研究以便更好地了解其对 Eimeria 的作用模式。
