Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
J Hazard Mater. 2012 May 30;217-218:279-85. doi: 10.1016/j.jhazmat.2012.03.030. Epub 2012 Mar 20.
Although the release of intracellular toxins after chlorination has been reported, the relation between cell lysis and the release and degradation of toxins during chlorination has not been well illustrated. This study used Microcystis aeruginosa to investigate the release and degradation behaviors of toxins after its exposure to chlorine at different doses for prolonged contact time. Scanning electron microscopy (SEM) analysis indicates no considerable change in the morphology for most algae cells. However, the release of intracellular K(+) and intracellular organic matter (IOM) did occur, and the significant K(+) release of 63% was observed even at chlorine exposure as low as 0.8 mg/L min. The damage of cell membrane was faster than the release of intracellular MCLR at all chlorine doses. Extracellular MCLR accumulated as a result of the fact that intracellular MCLR released from damaged cells faster than it was degraded by chlorine, especially at low dose of chlorine (0.8 mg/L). A maximum level of extracellular toxin at a contact time of 60, 30 and 1 min was observed at chlorine doses of 1.2, 1.6 and 2.0mg/L, respectively. In addition, Cl(2) consumption (%), toxin release and degradation (%), and cells lysis (%) were well related.
虽然已经报道了氯化后细胞内毒素的释放，但细胞裂解与氯化过程中毒素的释放和降解之间的关系尚未得到很好的说明。本研究使用铜绿微囊藻来研究在不同剂量的氯长时间接触后，毒素在暴露于氯后的释放和降解行为。扫描电子显微镜（SEM）分析表明，大多数藻类细胞的形态没有明显变化。然而，确实发生了细胞内 K(+)和细胞内有机质（IOM）的释放，即使在氯暴露低至 0.8mg/L min 的情况下，也观察到了 63%的显著 K(+)释放。在所有氯剂量下，细胞膜的损伤都比细胞内 MCLR 的释放快。由于受损细胞释放的细胞内 MCLR 比氯降解得更快，因此细胞外 MCLR 积累，尤其是在低剂量的氯（0.8mg/L）下。在接触时间为 60、30 和 1 分钟时，分别在氯剂量为 1.2、1.6 和 2.0mg/L 时观察到细胞外毒素的最大水平。此外，Cl(2)消耗（%）、毒素释放和降解（%）以及细胞裂解（%）之间存在良好的相关性。
