用于从哺乳动物细胞中提取蛋白质的冻融裂解。
Freeze-thaw lysis for extraction of proteins from Mammalian cells.
作者信息
Tansey William P
机构信息
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
出版信息
CSH Protoc. 2006 Dec 1;2006(7):pdb.prot4614. doi: 10.1101/pdb.prot4614.
PMID:22484670
Abstract
INTRODUCTIONThis protocol describes the extraction of proteins from mammalian cells grown in 6- or 10-cm tissue-culture dishes. It is useful for making very concentrated extracts for Western blotting, and is particularly helpful when low-abundance proteins such as Myc are being studied.
摘要
引言 本方案描述从在6厘米或10厘米组织培养皿中生长的哺乳动物细胞中提取蛋白质的方法。它对于制备用于蛋白质印迹法的高浓度提取物很有用，在研究诸如Myc等低丰度蛋白质时尤其有帮助。
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