Mathew Deepu George, Skariah K Skariah, Ranganathan Kannan
Department of Oral and Maxillofacial Pathology, Ragas Dental College and Hospital, Chennai, India.
Indian J Dent Res. 2011 Nov-Dec;22(6):879. doi: 10.4103/0970-9290.94693.
To isolate, culture and characterize fibroblasts from the buccal mucosa of areca nut chewers with and without oral submucous fibrosis (OSF).
Primary fibroblast cultures were established by the collagenase disaggregation technique and the phenotypic and growth characteristics were studied.
Cells cultured from OSF showed a statistically significant increase in both the post-mitotic fibroblast subpopulation and the population doubling time when compared with controls.
There was a significant increase in the pro-fibrotic, post-mitotic subpopulation of fibroblasts in areca nut chewers with OSF.
从有和没有口腔黏膜下纤维化（OSF）的槟榔咀嚼者的颊黏膜中分离、培养并鉴定成纤维细胞。
采用胶原酶解离技术建立原代成纤维细胞培养体系，并研究其表型和生长特性。
与对照组相比，从OSF患者培养的细胞在有丝分裂后成纤维细胞亚群和群体倍增时间上均有统计学显著增加。
患有OSF的槟榔咀嚼者中，促纤维化、有丝分裂后的成纤维细胞亚群显著增加。
