口腔黏膜下纤维化来源的口腔成纤维细胞的表型表达：通过细胞培养进行的分析
Phenotypic Expression of Oral Fibroblasts Derived from Oral Submucous Fibrosis: An Assay through Cell Culture.
作者信息
Banerjee Abhishek, Mampilly Mathew O, Kamath V V, Athreya Vijayaraghavan, Kotrashetti Vijayalakshmi, Srivastava Kumar Chandan, Shrivastava Deepti
机构信息
Department of Oral and Maxillofacial Pathology, Awadh Dental College Hospital, Jamshedpur, Jharkhand, India.
Department ofOral and Maxillofacial Surgery and Dentistry, ESIC Medical College and PG Institute, Bengaluru, Karnataka, India.
出版信息
J Pharm Bioallied Sci. 2021 Nov;13(Suppl 2):S1234-S1239. doi: 10.4103/jpbs.jpbs_408_21. Epub 2021 Nov 10.
OBJECTIVE
The present study aimed to establish cell lines of fibroblast from human OSF tissues and their response to varying concentrations of arecoline. The various morphological forms of fibroblasts were identified to establish phenotypic change.
MATERIALS AND METHOD
Fibroblast cell lines were obtained from control samples as well as from OSF cases. The cell lines were treated with 50/100/150/300/500 ug/ml of arecoline and morphology were determined.
RESULTS
Three morphological forms were detected; F1 spindle, F2 epitheloid and the F3 stellate. The F3 to F1 ratio was higher in OSF. Arecoline at 50ug/ml was stimulatory and at 150ug/ml cytotoxic to the cell lines.
CONCLUSION
Arecoline seems to enhance proliferation of the fibroblast at lower concentrations but cytotoxic at higher levels. This is probably due to the generation of new cell lines and response of the arecoline receptors indicating phenotypic change.
目的
本研究旨在建立人口腔黏膜下纤维化（OSF）组织的成纤维细胞系及其对不同浓度槟榔碱的反应。鉴定成纤维细胞的各种形态形式以确定表型变化。
材料与方法
从对照样本以及OSF病例中获取成纤维细胞系。用50/100/150/300/500微克/毫升的槟榔碱处理细胞系并确定形态。
结果
检测到三种形态形式；F1梭形、F2上皮样和F3星状。OSF中F3与F1的比例更高。50微克/毫升的槟榔碱对细胞系有刺激作用，而150微克/毫升时具有细胞毒性。
结论
槟榔碱在较低浓度下似乎能增强成纤维细胞的增殖，但在较高浓度时具有细胞毒性。这可能是由于新细胞系的产生以及槟榔碱受体的反应表明了表型变化。
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