Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Z Naturforsch C J Biosci. 2012 Jan-Feb;67(1-2):47-57. doi: 10.1515/znc-2012-1-207.
The protective and therapeutic effects of Argyreia speciosa Sweet (Convolvulaceae) against ethanol-induced gastric ulcer in rats were evaluated. Ethanolic and water extracts of the aerial plant parts (200 mg/kg body weight) were orally administered daily for seven days prior to or after ulceration with one oral dose of 1 mL absolute ethanol on 24-h empty stomachs. Rats were divided into eleven groups. Group 1 served as control. To groups 2 and 3 each extract was administered. Groups 4 to 6 received each extract or ranitidine (100 mg/kg body weight) prior to ulcer induction. Groups 7 to 9 received each extract or ranitidine post ulcer induction. Groups 10 and 11 were gastric ulcerative rats after one hour and one week of ethanol induction. The evaluation was done through measuring ulcer indices: stomach acidity and volume, lesion counts, mucus, and prostaglandin E2 contents. Oxidative stress marker, i. e. malondialdehyde, glutathione, and superoxide dismutase, were estimated. Certain marker enzymes for different cell organelles, i. e. succinate and lactate dehydrogenases, glucose-6-phosphatase, acid phosphatase, and 5'-nucleotidase, were evaluated. The work was extended to determine the collagen content and the histopathological assessment of the stomach. Gastric ulcer exhibited a significant elevation of the ulcer index, antioxidant levels, collagen content, and the marker enzymes. The water extract attenuated these increments and was more potent as a protective agent, while the ethanol extract exhibited stronger therapeutic potency. In conclusion, A. speciosa acted as antiulcer agent. More detailed studies are required to identify the compounds responsible for the pharmacological effect.
评价山芝麻（旋花科）对乙醇诱导的大鼠胃溃疡的保护和治疗作用。将地上部分的乙醇和水提取物（200mg/kg 体重）在溃疡前或溃疡后 24 小时空腹时给予大鼠口服，每日一次，连续 7 天，每次给予 1ml 无水乙醇。将大鼠分为 11 组。第 1 组作为对照。第 2 组和第 3 组分别给予各提取物。第 4 组至第 6 组在诱导溃疡前给予各提取物或雷尼替丁（100mg/kg 体重）。第 7 组至第 9 组在诱导溃疡后给予各提取物或雷尼替丁。第 10 组和第 11 组为乙醇诱导 1 小时和 1 周后发生胃溃疡的大鼠。通过测量溃疡指数：胃酸度和容量、病变计数、黏液和前列腺素 E2 含量来进行评估。估计了氧化应激标志物，即丙二醛、谷胱甘肽和超氧化物歧化酶。评估了不同细胞器的某些标记酶，即琥珀酸和乳酸脱氢酶、葡萄糖-6-磷酸酶、酸性磷酸酶和 5'-核苷酸酶。这项工作扩展到确定胃的胶原含量和组织病理学评估。胃溃疡表现出溃疡指数、抗氧化剂水平、胶原含量和标记酶的显著升高。水提取物减轻了这些增加，作为保护剂更有效，而乙醇提取物表现出更强的治疗效力。总之，山芝麻具有抗溃疡作用。需要进一步的研究来确定负责药理作用的化合物。
