Fahmi Abdelgawad A, Abdur-Rahman Mariam, Aboul Naser Asmaa F, Hamed Manal A, Abd-Alla Howaida I, Shalaby Nagwa M M, Nasr Mohamed I
Chemistry Department, Faculty of Science, Cairo University, Cairo, Egypt.
Department of Therapeutic Chemistry, National Research Centre, Giza, Egypt.
Heliyon. 2019 Mar 20;5(3):e01359. doi: 10.1016/j.heliyon.2019.e01359. eCollection 2019 Mar.
subsp. (Family; Asteraceae) is a medicinal plant used to treat inflammation. The objective of this study is to explore the protective effect of the ethanol extract of subsp. aerial parts against ethanol induced gastric ulcer in rats. The chemical composition of plant extract, the unsaponifiable matter and the fatty acid methyl esters were analyzed. The biological evaluation was carried out through measuring ulcer indices, oxidative stress markers, certain marker enzymes, inflammatory index and the histopathological assessment of the stomach in rats. The total unsaponifiable matter (94.29%) and the fatty acid methyl ester (82.96%) content were identified. Gastric ulcer recorded significant increase in gastric volume and lesion counts (p < 0.0001). Drastic changes in all biochemical parameters under investigation were observed. Protection with plant extract reversed the action of ethanol by variable degrees of improvement in comparison with the reference drug. The presence of carbohydrates and proteins that acted as a mucilage lining the stomach inner wall give its protective action. In conclusion, subsp. succeeded to have anti-ulcerative protective effect. The measured biomarkers served as a good mirror to predict gastric ulcer and the presence of carbohydrates, protein and fibers present in the plant extract acted as a mucilage lining the inner intestinal wall and protect against ethanol induced gastric ulcer. Future study will be carried out to identify the biologically active compounds responsible for plant protection against the gastric ulcer.
亚种（菊科）是一种用于治疗炎症的药用植物。本研究的目的是探讨该亚种地上部分乙醇提取物对大鼠乙醇诱导胃溃疡的保护作用。分析了植物提取物的化学成分、不皂化物和脂肪酸甲酯。通过测量溃疡指数、氧化应激标志物、某些标志物酶、炎症指数以及对大鼠胃进行组织病理学评估来进行生物学评价。确定了总不皂化物含量（94.29%）和脂肪酸甲酯含量（82.96%）。胃溃疡大鼠的胃体积和损伤计数显著增加（p < 0.0001）。观察到所有研究的生化参数都有剧烈变化。与参比药物相比，植物提取物的保护作用通过不同程度的改善逆转了乙醇的作用。植物提取物中作为黏液衬于胃内壁的碳水化合物和蛋白质的存在赋予了其保护作用。总之，该亚种成功地具有抗溃疡保护作用。所测量的生物标志物是预测胃溃疡的良好指标，并且植物提取物中存在的碳水化合物、蛋白质和纤维作为黏液衬于肠道内壁，保护大鼠免受乙醇诱导的胃溃疡。未来将开展研究以鉴定负责植物抗胃溃疡保护作用的生物活性化合物。
