a Faculty of Science, Chemistry Department , Cairo University , Cairo , Egypt.
b Department of Therapeutic Chemistry , National Research Centre , Giza , Egypt.
Biomarkers. 2019 May;24(3):286-294. doi: 10.1080/1354750X.2018.1556340. Epub 2019 Jan 14.
Stomach ulcers are the common gastrointestinal disorders worldwide. This study aimed to investigate the therapeutic impact of aerial parts ethanol extract against gastric ulcer in rats. Ulcer was induced by one oral dose of ethanol (0.5 ml/100g body weight) on 24 hours empty stomach, then the plant extract (500 mg/kg b.wt.) was orally administered daily for one week. Ranitidine (100 mg/kg b.wt.); as a reference drug was evaluated. Stomach acidity and volume, as well as lesion counts were measured. Levels of malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) were estimated. Assay of different marker enzymes; succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), glucose-6-phosphatase (G-6-Pase), acid phosphatase (AP) and 5'-nucleotidase (5'NT) were determined. Interlukin-10 (IL-10), intracellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor alpha (TNF-α) were also determined. Stomach histopathological assessment was detected. : Gastric ulcer showed drastic changes in oxidative stress, cell organelles and inflammatory markers. These biomarkers served as good tools to identify the presence of gastric ulcer. Treatment with recorded amelioration in most parameters exceeding the auto healing effect. Healing potency of is possibly related to its content of glycosides, coumarins, flavonoids, tannins, sterols and triterpenes.
胃溃疡是全球常见的胃肠道疾病。本研究旨在探讨地上部分乙醇提取物对大鼠胃溃疡的治疗作用。溃疡通过在 24 小时空腹时口服一剂乙醇（0.5ml/100g 体重）诱导，然后每天口服植物提取物（500mg/kg 体重）持续一周。雷尼替丁（100mg/kg 体重）；作为参考药物进行评估。测量胃酸和容量以及病变计数。测定丙二醛（MDA）、谷胱甘肽（GSH）和超氧化物歧化酶（SOD）的水平。测定不同标记酶；琥珀酸脱氢酶（SDH）、乳酸脱氢酶（LDH）、葡萄糖-6-磷酸酶（G-6-Pase）、酸性磷酸酶（AP）和 5'-核苷酸酶（5'NT）。还测定了白细胞介素-10（IL-10）、细胞间黏附分子-1（ICAM-1）和肿瘤坏死因子-α（TNF-α）。检测胃组织病理学评估。：胃溃疡在氧化应激、细胞器和炎症标志物方面发生了剧烈变化。这些生物标志物是识别胃溃疡存在的良好工具。与自动愈合作用相比，治疗组在大多数参数上均有改善。的愈合能力可能与其糖苷、香豆素、类黄酮、单宁、甾醇和三萜的含量有关。
