Kirk K
University Laboratory of Physiology, Oxford, UK.
NMR Biomed. 1990 Feb;3(1):1-16. doi: 10.1002/nbm.1940030102.
NMR methods for measuring membrane transport rates rely in most cases upon there being a significant transmembrane difference in either the resonance frequency or the NMR relaxation times of the transported species. The first part of this review examines the various factors that can give rise to such differences in cell and vesicle suspensions. The second part provides an introduction to the theory of membrane transport measurements and describes the different ways in which transmembrane differences in NMR parameters have been exploited in monitoring both slow and fast membrane transport processes.
用于测量膜转运速率的核磁共振（NMR）方法在大多数情况下依赖于被转运物质的共振频率或NMR弛豫时间在跨膜方向上存在显著差异。本综述的第一部分探讨了在细胞和囊泡悬浮液中可能导致这种差异的各种因素。第二部分介绍了膜转运测量的理论，并描述了利用NMR参数的跨膜差异来监测慢速和快速膜转运过程的不同方法。
