Kuchel P W
Department of Biochemistry, University of Sydney, NSW, Australia.
NMR Biomed. 1990 Jun;3(3):102-19. doi: 10.1002/nbm.1940030303.
Spin-exchange NMR techniques enable the measurement of the rates of exchange of solutes between chemically or physically distinct sites in reactions taking place at chemical equilibrium. The time scale of the events that are able to be investigated lies in the neighbourhood of 1 s. The earliest studies in this area of NMR spectroscopy involved chemical reactions in vitro but the procedures have been adapted to the study of enzyme-catalysed reactions both in vitro and in vivo, and more recently to transmembrane exchange processes. The emphasis in this review is on the various types of spin-exchange experiments, the analysis of data derived from them, estimates of uncertainty in measured rate constants, and their shortcomings. Those methods given special attention are saturation transfer, two-dimensional exchange spectroscopy (2D EXSY), the 'accordion' experiment and 'overdetermined' one-dimensional exchange spectroscopy.
自旋交换核磁共振技术能够测量处于化学平衡的反应中，溶质在化学或物理上不同位点之间的交换速率。能够被研究的事件的时间尺度约为1秒。核磁共振光谱学这一领域最早的研究涉及体外化学反应，但这些方法已被应用于体外和体内酶催化反应的研究，最近还应用于跨膜交换过程。本综述重点关注各种类型的自旋交换实验、从这些实验中获得的数据的分析、测量速率常数的不确定性估计以及它们的缺点。受到特别关注的方法有饱和转移、二维交换光谱法（2D EXSY）、“手风琴”实验和“超定”一维交换光谱法。
