Department of Analytical Chemistry, Chemical Faculty, Silesian University of Technology, 7 Strzody Str., 44-100, Gliwice, Poland.
Bull Environ Contam Toxicol. 2012 Jul;89(1):8-14. doi: 10.1007/s00128-012-0634-7. Epub 2012 Apr 10.
A SPE-UHPLC-UV method for the determination of 13 pharmaceuticals from different therapeutic groups in surface water and wastewater samples was proposed. The following three solid-phase-extraction (SPE) columns with polymeric sorbents were used as a pre-concentration step: the Oasis HLB (average recoveries 93.8 %), the Nexus (84.0 %) and the Bond Elut ENV (88.3 %). A reverse-phase UHPLC with a C(18e) monolithic column and gradient elution program was used to obtain the best separations for all 13 drugs in short analysis time (3.4 min). The LOD range for determined drugs was 0.02-0.18 μg L(-1), and the concentration range for drugs found in water samples was 0.06-0.90 μg L(-1). The proposed method was used to analyze different water samples, mostly from rivers, and can be used as a monitoring tool for environmental pollution.
提出了一种 SPE-UHPLC-UV 方法，用于测定地表水和废水中 13 种不同治疗组别的药物。采用三种带有聚合物吸附剂的固相萃取（SPE）柱作为预浓缩步骤：Oasis HLB（平均回收率 93.8%）、Nexus（84.0%）和 Bond Elut ENV（88.3%）。反相 UHPLC 采用 C(18e)整体柱和梯度洗脱程序，可在短分析时间（3.4 分钟）内实现所有 13 种药物的最佳分离。所测定药物的 LOD 范围为 0.02-0.18μg L(-1)，在水样中发现的药物浓度范围为 0.06-0.90μg L(-1)。该方法已用于分析来自河流等不同水源的水样，可作为环境污染监测工具。
