Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China.
Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan.
Oxid Med Cell Longev. 2020 Feb 10;2020:9585047. doi: 10.1155/2020/9585047. eCollection 2020.
Quercetin, a flavonoid found in fruits and vegetables, is widely distributed as a secondary metabolite in the plant kingdom. Oxidative stress plays a role in the pathogenesis of diabetes mellitus (DM). The present study investigated the effects of quercetin dietary supplementation on streptozotocin- (STZ-) induced hyperglycemic Arbor Acre (AA) broilers by determining the levels of fasting blood glucose (FBG), fasting insulin (FINS), biochemical indicators, oxidative stress markers, inflammatory cytokines content, antioxidant enzymes activities in tissues, and mRNA expression of genes relating to the insulin signaling pathway. Three hundred one-day-old healthy AA broilers were randomly assigned into 5 treatments; A, control healthy broilers; B, STZ-induced broilers; C, STZ-induced broiler dietary supplemented with 0.02% quercetin; D, STZ-induced broiler dietary supplemented with 0.04% quercetin; and E, STZ-induced broiler dietary supplemented with 0.06% quercetin. The results showed that quercetin supplementation relieved the side effects of STZ-induced oxidative stress by changing activities of antioxidant enzymes, decreasing malondialdehyde (MDA) and nitric oxide (NO) levels, activating expression of genes relating to PI3K/PKB signaling pathway that modulate glucose metabolism and reduce oxidative damage, thereby decreasing FBG and increasing FINS levels. These findings suggest that quercetin exhibits a protective effect in STZ-induced hyperglycemic AA broilers via decreasing oxidative stress.
槲皮素是一种存在于水果和蔬菜中的类黄酮，广泛分布于植物界作为次生代谢物。氧化应激在糖尿病（DM）的发病机制中起作用。本研究通过测定空腹血糖（FBG）、空腹胰岛素（FINS）、生化指标、氧化应激标志物、组织中炎症细胞因子含量、抗氧化酶活性以及与胰岛素信号通路相关的基因的 mRNA 表达水平，研究了槲皮素饮食补充对链脲佐菌素（STZ）诱导的高血糖 Arbor Acre（AA）肉鸡的影响。301 日龄健康 AA 肉鸡随机分为 5 组处理；A、对照组健康肉鸡；B、STZ 诱导的肉鸡；C、STZ 诱导的肉鸡补充 0.02%槲皮素；D、STZ 诱导的肉鸡补充 0.04%槲皮素；E、STZ 诱导的肉鸡补充 0.06%槲皮素。结果表明，槲皮素通过改变抗氧化酶的活性、降低丙二醛（MDA）和一氧化氮（NO）水平、激活与 PI3K/PKB 信号通路相关的基因的表达来缓解 STZ 诱导的氧化应激的副作用，这些基因调节葡萄糖代谢并减少氧化损伤，从而降低 FBG 并提高 FINS 水平。这些发现表明，槲皮素通过降低氧化应激对 STZ 诱导的高血糖 AA 肉鸡表现出保护作用。
