State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China.
BMC Microbiol. 2012 Apr 11;12:54. doi: 10.1186/1471-2180-12-54.
Enzybiotics are becoming increasingly recognized as potential alternative therapies for drug-resistant bacteria. Although only a few enzybiotics are currently well characterized, much information is still missing or is unavailable for researchers. The construction of an enzybiotics database would therefore increase efficiency and convenience in investigating these bioactive proteins and thus help reduce or delay the recent increase in antibiotic resistance.
In the present manuscript, we describe the development of a novel and original database called EnzyBase, which contains 1144 enzybiotics from 216 natural sources. To ensure data quality, we limited the source of information to authoritative public databases and published scientific literature. The interface of EnzyBase is easy to use and allows users to rapidly retrieve data according to their desired search criteria and blast the database for homologous sequences. We also describe examples of database-aided enzybiotics discovery and design.
EnzyBase serves as a unique tool for enzybiotic studies. It has several potential applications, e.g. in silico enzybiotic combination as cocktails, and novel enzybiotic design, in response to continuously emerging drug-resistant pathogens. This database is a valuable platform for researchers who are interested in enzybiotic studies. EnzyBase is available online at http://biotechlab.fudan.edu.cn/database/EnzyBase/home.php.
越来越多的人认识到，酶原生物制剂可能成为治疗耐药菌的替代疗法。虽然目前只有少数几种酶原生物制剂得到了很好的描述，但仍有很多信息缺失或无法供研究人员使用。因此，构建一个酶原生物制剂数据库将提高研究这些生物活性蛋白的效率和便利性，从而有助于减少或延缓最近抗生素耐药性的增加。
在本手稿中，我们描述了一种新型的原始数据库 EnzyBase 的开发，该数据库包含了来自 216 个天然来源的 1144 种酶原生物制剂。为了确保数据质量，我们将信息来源仅限于权威的公共数据库和已发表的科学文献。EnzyBase 的界面易于使用，允许用户根据所需的搜索条件快速检索数据，并对数据库进行同源序列比对。我们还描述了一些基于数据库的酶原生物制剂发现和设计的示例。
EnzyBase 是一种用于酶原生物制剂研究的独特工具。它有几个潜在的应用，例如用于模拟的酶原生物制剂组合成鸡尾酒，以及用于设计新的酶原生物制剂，以应对不断出现的耐药性病原体。该数据库是对酶原生物制剂研究感兴趣的研究人员的宝贵平台。EnzyBase 可在线访问，网址为 http://biotechlab.fudan.edu.cn/database/EnzyBase/home.php。
