Department of Parasitology, Leiden University Medical Center, The Netherlands.
Am J Trop Med Hyg. 2012 Apr;86(4):613-9. doi: 10.4269/ajtmh.2012.11-0463.
This study investigated in utero priming as a consequence of maternal parasitic infections. Cord blood plasma samples of 63 African newborns were assessed by enzyme-linked immunosorbent assay for their content of total and schistosome-specific or filaria-specific IgE and IgG4. The frequencies of lymphocyte phenotypes in cord blood were also determined by using flow cytometry, and were compared with those of European newborns. We found significantly increased schistosome soluble egg antigen (SEA)-specific IgE in cord plasma of those born to mothers with schistosome infections and correlations between fetal and maternal SEA-specific and filaria antigen-specific IgE. These data are evidence for in utero priming of the fetal immune system to maternal helminth infections. Furthermore, we show significantly enhanced percentages of CD5- B cells in African newborns cord blood compared with Europeans, which is consistent with earlier maturation of the African fetal immune system.
本研究探讨了母体寄生虫感染导致的宫内致敏现象。通过酶联免疫吸附试验，对 63 名非洲新生儿的脐血血浆样本进行了总 IgE、血吸虫特异性 IgE 和丝虫特异性 IgE、IgG4 的检测。还通过流式细胞术测定了脐血淋巴细胞表型的频率，并与欧洲新生儿进行了比较。我们发现，来自有血吸虫感染母亲的新生儿脐血血浆中，血吸虫可溶性卵抗原（SEA）特异性 IgE 显著增加，并且胎儿和母体 SEA 特异性及丝虫抗原特异性 IgE 之间存在相关性。这些数据为胎儿免疫系统对母体寄生虫感染的宫内致敏提供了证据。此外，与欧洲人相比，我们还发现非洲新生儿脐带血中 CD5-B 细胞的百分比显著增加，这与非洲胎儿免疫系统的早期成熟相一致。
