Department of Cranio-Maxillo-Facial Surgery, University Medical Center Regensburg, Germany.
J Biomater Appl. 2013 Aug;28(2):187-98. doi: 10.1177/0885328212441957. Epub 2012 Apr 5.
Staphylococcus epidermidis plays a major role in capsular contractures of silicone breast implants. This in vitro study evaluates the antibacterial effect of copper on S. epidermidis in silicone implants. Specimens of a silicone material used for breast augmentation (Cu0) and specimens coated with different copper concentrations (Cu1, Cu2) were artificially aged. Surface roughness and surface free energy were assessed. The specimens were incubated in an S. epidermidis suspension. We assessed the quantification and the viability of adhering bacteria by live/dead cell labeling with fluorescence microscopy. Additionally, inhibition of bacterial growth was evaluated by agar diffusion, broth culture, and quantitative culture of surface bacteria. No significant differences in surface roughness and surface free energy were found between Cu0, Cu1 and Cu2. Aging did not change surface characteristics and the extent of bacterial adhesion. Fluorescence microscopy showed that the quantity of bacteria on Cu0 was significantly higher than that on Cu1 and Cu2. The ratio of dead to total adhering bacteria was significantly lower on Cu0 than on Cu1 and Cu2, and tended to be higher for Cu2 than for Cu1. Quantitative culture showed equal trends. Copper additives seem to have anti-adherence and bactericidal effects on S. epidermidis in vitro.
表皮葡萄球菌在硅酮乳房植入物胶囊挛缩中起主要作用。本体外研究评估了铜对硅酮植入物中表皮葡萄球菌的抗菌作用。使用用于乳房增大的硅材料标本（Cu0）和用不同铜浓度（Cu1、Cu2）涂布的标本进行人工老化。评估表面粗糙度和表面自由能。将标本在表皮葡萄球菌悬浮液中孵育。通过荧光显微镜的活/死细胞标记评估粘附细菌的定量和活力。此外，通过琼脂扩散、肉汤培养和表面细菌定量培养评估细菌生长的抑制作用。Cu0、Cu1 和 Cu2 之间的表面粗糙度和表面自由能没有显著差异。老化不会改变表面特性和细菌粘附的程度。荧光显微镜显示 Cu0 上的细菌数量明显高于 Cu1 和 Cu2。Cu0 上的死菌与总粘附菌的比例明显低于 Cu1 和 Cu2，而 Cu2 上的比例高于 Cu1。定量培养显示出相同的趋势。铜添加剂似乎对表皮葡萄球菌具有体外抗粘附和杀菌作用。
