定量 PCR 跟踪模型鞘氨醇单胞菌中巨质粒携带的生物降解潜能。
Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad.
机构信息
The Swette Center for Environmental Biotechnology, The Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
出版信息
Appl Environ Microbiol. 2012 Jun;78(12):4493-6. doi: 10.1128/AEM.00715-12. Epub 2012 Apr 6.
Abstract
We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenase-encoding genes.
摘要
我们开发了一种定量 PCR 方法来追踪 Sphingomonas wittichii RW1 的二苯并-p-二恶英降解途径中最初的、大质粒携带的 dxnA1 基因。我们在复杂的环境样本中使用了这种方法，并报告了 S. wittichii RW1 在垃圾渗滤液中的生长情况，从而为监测大质粒携带的双加氧酶编码基因提供了一种新工具。
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