CSIRO Materials Science and Engineering, Clayton South, VIC 3169, Australia.
J Biomed Mater Res A. 2012 Sep;100(9):2239-43. doi: 10.1002/jbm.a.34164. Epub 2012 Apr 10.
Photocrosslinking, using 2 mM Ru(II)(bpy)(3)Cl(2) and various concentrations of sodium persulfate with irradiation by blue light, ∼455 nm, has been shown to be a rapid and effective method for crosslinking various tissues: tendon, amnion membrane, pericardium, and heart valve leaflet. The presence of new crosslinking was demonstrated by the increase in the shrinkage temperature of these tissues. In all the cases, increase in the shrinkage temperatures were seen, although at higher sodium persulfate concentrations, for example, 100 mM, both with and without the Ru(II)(bpy)(3)Cl(2) catalyst, some degradation of the collagenous tissues was found. The effectiveness of this photocrosslinking method when used with tissues was also shown through the increase in the break strength of tissues after crosslinking, and by the reduction of protein that could be extracted by urea. In solution studies, dityrosine has been shown to be formed during photocrosslinking. With tissues, Western blotting showed the presence of new dityrosine crosslinked proteins.
光交联，使用 2mM Ru(II)(bpy)(3)Cl(2) 和不同浓度的过硫酸钠，用蓝色光（约 455nm）照射，已被证明是一种快速有效的交联各种组织的方法：肌腱、羊膜膜、心包和心脏瓣膜。这些组织的收缩温度的增加证明了新交联的存在。在所有情况下，尽管在较高浓度的过硫酸钠（例如 100mM）下，无论是使用还是不使用 Ru(II)(bpy)(3)Cl(2)催化剂，都发现一些胶原组织发生了降解，但收缩温度都有所升高。通过交联后组织断裂强度的增加以及通过脲提取的蛋白质减少，也表明了这种光交联方法在组织中的有效性。在溶液研究中，已经证明光交联过程中形成了二酪氨酸。通过对组织进行 Western blot 分析，显示存在新的二酪氨酸交联蛋白。
