丹麦一个偏远岛屿上野生水貂中阿留申水貂病病毒的高流行率。

High prevalence of Aleutian mink disease virus in free-ranging mink on a remote Danish island.

作者信息

Jensen Trine H, Christensen Laurids S, Chriél Mariann, Harslund Jakob, Salomonsen Charlotte M, Hammer Anne Sofie

机构信息

Division of Poultry, Fish, and Fur Animals, National Veterinary Institute, Technical University of Denmark, Hangøvej 2, DK-8200 Aarhus, Denmark.

出版信息

J Wildl Dis. 2012 Apr;48(2):497-502. doi: 10.7589/0090-3558-48.2.497.

DOI:10.7589/0090-3558-48.2.497

PMID:22493130

Abstract

Aleutian mink disease virus (AMDV) causes severe disease in farmed mink (Neovison vison) worldwide. In Denmark, AMDV in farmed mink has been confined to the northern part of the mainland since 2002. From 1998 to 2009, samples from 396 free-ranging mink were collected from mainland Denmark, and a low AMDV antibody prevalence (3% of 296) was found using countercurrent immune electrophoresis. However, on the island of Bornholm in the Baltic Sea, a high prevalence (45% of 142 mink) was detected in the free-ranging mink. Aleutian mink disease virus was detected by polymerase chain reaction in 32 of 49 antibody-positive free-ranging mink on Bornholm, but not in mink collected from other parts of Denmark. Sequence analysis of 370 base pairs of the nonstructural gene of the AMDV of 17 samples revealed two clusters with closest similarity to Swedish AMDV strains.

摘要

阿留申水貂病病毒（AMDV）在全球范围内的养殖水貂（Neovison vison）中引发严重疾病。在丹麦，自2002年以来，养殖水貂中的AMDV一直局限于本土北部地区。1998年至2009年期间，从丹麦本土采集了396只野生水貂的样本，通过对流免疫电泳发现AMDV抗体阳性率较低（296只中有3%）。然而，在波罗的海的博恩霍尔姆岛上，野生水貂中检测到高阳性率（142只水貂中有45%）。通过聚合酶链反应在博恩霍尔姆岛49只抗体阳性野生水貂中的32只检测到了阿留申水貂病病毒，但在从丹麦其他地区采集的水貂中未检测到。对17个样本的AMDV非结构基因370个碱基对进行序列分析，发现两个与瑞典AMDV毒株相似度最高的簇。