Department of Animal Science and Aquaculture, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia, B2N 5E3, Canada.
Perennia Food and Agriculture, Bible Hill, Nova Scotia, B4N 1J5, Canada.
BMC Vet Res. 2020 Nov 30;16(1):465. doi: 10.1186/s12917-020-02685-w.
Feed additives which can ease the negative effects of infection by the Aleutian mink disease virus (AMDV) are of interest to mink farmers. The effects of kelp meal (Ascophylum nodosum) supplementation on immune response, virus replication and blood parameters of mink inoculated with AMDV were assessed. AMDV-free black mink (n = 75) were intranasally inoculated with a local strain of AMDV and fed a commercial pellet supplemented with kelp meal at the rates of 1.5% or 0.75% of the feed or were kept as controls (no kelp) for 451 days. Blood was collected on days 0 (pre-inoculation), 31, 56, 99, 155, 366 and 451 post-inoculation (dpi).
No significant difference was observed among the treatments for the proportion of animals positive for antibodies against the virus measured by the counter-immunoelectrophoresis (CIEP), viremia measured by PCR, antibody titer measured by quantitative ELISA, total serum protein measured by a refractometer or elevated levels of gamma globulin measured by iodine agglutination test at the sampling occasions. At the termination of the experiment on 451 dpi, there were no differences among treatments for antibody titer measured by CIEP, total serum protein, albumin, globulins, albumin:globulin ratio, alkaline phosphatase, gamma-glutamyl transferase, and proportions of PCR positive spleen, lymph node or bone marrow samples, but blood urea nitrogen and creatine levels were significantly lower in the 1.5% kelp supplemented group than in the controls.
Kelp supplementation improved kidney function of mink infected with AMDV with no effect on liver function, immune response to infection by AMDV or virus replication.
能够缓解传染性貂阿留申病病毒（AMDV）感染负面影响的饲料添加剂是水貂养殖户感兴趣的。本研究评估了海带粉（Ascophylum nodosum）补充剂对感染 AMDV 的水貂免疫反应、病毒复制和血液参数的影响。将 75 只无 AMDV 的黑色水貂（n = 75）经鼻腔接种当地 AMDV 株，并饲喂商业颗粒料，其中添加 1.5%或 0.75%的海带粉作为补充，或作为对照组（不添加海带），持续 451 天。在接种前（0 dpi）、31、56、99、155、366 和 451 dpi 采集血液。
在通过对流免疫电泳（CIEP）测量的针对病毒的抗体阳性动物比例、通过 PCR 测量的病毒血症、通过定量 ELISA 测量的抗体滴度、通过折射仪测量的总血清蛋白或通过碘凝集试验测量的升高的γ球蛋白水平方面，各处理组之间没有显著差异。在 451 dpi 实验结束时，CIEP 测量的抗体滴度、总血清蛋白、白蛋白、球蛋白、白蛋白：球蛋白比值、碱性磷酸酶、γ-谷氨酰转移酶以及脾脏、淋巴结或骨髓样本中 PCR 阳性的比例在各组之间没有差异，但 1.5%海带补充组的血尿素氮和肌酸水平显著低于对照组。
海带补充剂改善了感染 AMDV 的水貂的肾功能，但对肝功能、对 AMDV 感染的免疫反应或病毒复制没有影响。
