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多重 PCR 快速改进肝移植受者血流感染诊断

Multiplex PCR for rapid and improved diagnosis of bloodstream infections in liver transplant recipients.

机构信息

Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.

出版信息

J Clin Microbiol. 2012 Jun;50(6):2069-71. doi: 10.1128/JCM.00745-12. Epub 2012 Apr 4.

DOI:10.1128/JCM.00745-12
PMID:22493334
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3372100/
Abstract

This prospective study evaluated the utility of the SeptiFast (SF) test in detecting 25 clinically important pathogens in 225 blood samples from 170 intensive care unit (ICU) patients with suspected sepsis after liver transplantation (LTX) or after other major abdominal surgery (non-LTX). SF yielded a significantly higher positivity rate in the LTX group (52.3%) than in the non-LTX group (30.5%; P = 0.0009). SF may be a powerful tool for the early diagnosis of bloodstream infections in LTX patients.

摘要

这项前瞻性研究评估了 SeptiFast（SF）检测在检测 170 名疑似肝移植（LTX）或其他主要腹部手术后重症监护病房（ICU）患者 225 份血液样本中 25 种临床重要病原体中的效用。SF 在 LTX 组中的阳性率（52.3%）明显高于非 LTX 组（30.5%；P = 0.0009）。SF 可能是 LTX 患者血流感染早期诊断的有力工具。

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本文引用的文献

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J Clin Microbiol. 2011 Jun;49(6):2252-8. doi: 10.1128/JCM.02460-10. Epub 2011 Apr 6.
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Fungal Infections After Liver Transplantation.肝移植后的真菌感染
Transplant Proc. 1988 Feb 1;20(1):650-651.
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Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit.采用脓毒症病原体 DNA 检测试剂盒的实时聚合酶链反应法对临床样本中病原体检测的评估。
Crit Care. 2010;14(4):R159. doi: 10.1186/cc9234. Epub 2010 Aug 24.
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Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia.多重血液 PCR 联合血液培养在发热性中性粒细胞减少症中改善感染的微生物学记录。
J Clin Microbiol. 2010 Oct;48(10):3510-6. doi: 10.1128/JCM.00147-10. Epub 2010 Aug 18.
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Epidemiology and outcome of invasive fungal infections in solid organ transplant recipients.实体器官移植受者侵袭性真菌感染的流行病学及结局
Transpl Infect Dis. 2010 Jun;12(3):220-9. doi: 10.1111/j.1399-3062.2010.00492.x. Epub 2010 Jan 25.
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Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group.欧洲癌症研究与治疗组织/侵袭性真菌感染合作组和美国国立过敏与传染病研究所真菌病研究组（EORTC/MSG）共识组对侵袭性真菌病的修订定义。
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