Department of Orofacial Sciences, University of California at San Francisco, San Francisco, CA 94143-0422, USA.
Anticancer Res. 2012 Apr;32(4):1163-6.
We previously showed that within primary tumors there exist subpopulations of cells expressing stem cell markers. Using immunofluorescence and western blotting, we examined the expression of stem cell markers tumor-rejection antigen 1-60 (TRA1-60) and octamer-binding transcription factor 3/ 4 (OCT3/4) to determine their relationship with cell invasiveness. Six human oral cancer cell lines were examined and a direct correlation was found between expression of these stem cell markers and invasion. Poor expression of E-cadherin and increased expression of N-cadherin was also found in TRA1-60- and OCT3/4- expressing cells. Phosphorylation of the major signaling molecule mitogen activated protein kinase (MAPK) was greatest in the TRA1-60- and OCT3/4- expressing cells. These results suggest that expression of specific stem cell markers in tumors may help guide a clinician's choice of treatment.
我们之前曾表明，原发性肿瘤内存在表达干细胞标志物的细胞亚群。我们通过免疫荧光和 Western blot 检测了肿瘤排斥抗原 1-60（TRA1-60）和八聚体结合转录因子 3/4（OCT3/4）的表达，以确定它们与细胞侵袭性的关系。我们检测了 6 个人类口腔癌细胞系，发现这些干细胞标志物的表达与侵袭性之间存在直接相关性。在表达 TRA1-60 和 OCT3/4 的细胞中，E-钙黏蛋白表达水平较低，N-钙黏蛋白表达水平较高。促分裂原活化蛋白激酶（MAPK）这一主要信号分子的磷酸化在表达 TRA1-60 和 OCT3/4 的细胞中最为显著。这些结果表明，肿瘤中特定干细胞标志物的表达可能有助于指导临床医生选择治疗方案。
