Li Xi-Wen, Hu Zhi-Gang, Lin Xiao-Han, Li Qing, Gao Huan-Huan, Luo Guo-An, Chen Shi-Lin
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Yao Xue Xue Bao. 2012 Jan;47(1):124-30.
Chloroplast genome sequences have comprehensive application prospects in DNA barcoding and chloroplast engineering in traditional Chinese medicine. The complete chloroplast genome of Magnolia officinalis sequenced by high-throughput pyrosequencing and a sequencing procedure was established. Fourteen contigs were obtained after de nove assembly. The sequencing percent of coverage was 99.99%. The chloroplast genome is 160 183 bp in size, and has a typical quadripartite structure with the large (LSC, 88 210 bp) and small copy (SSC, 18 843 bp) regions separated by two copies of an inverted repeat (IRs, 26 565 bp each). chloroplast genes were successfully annotated, of which 17 genes located in each IR region. The chloroplast genome features in Magnolia officinalis are nearly identical to those from other Magnoliid chloroplast genomes. Phylogenetic analyses were performed based on 81 shared coding-genes for a total of 9 Magnolia samples of 5 closely related species. Results showed that distinguishing among species was generally straightforward at the species and population level. This study confirmed the effectiveness of our chloroplast genome sequencing procedure. The chloroplast genome can provide distinguishing differences to help identify Magnolia officinalis and its closely related plants.
叶绿体基因组序列在中药的DNA条形码和叶绿体工程方面具有广泛的应用前景。通过高通量焦磷酸测序对厚朴的叶绿体全基因组进行了测序，并建立了测序流程。从头组装后获得了14个重叠群。测序覆盖率为99.99%。叶绿体基因组大小为160183 bp，具有典型的四分体结构，其中大单拷贝区（LSC，88210 bp）和小单拷贝区（SSC，18843 bp）被两个反向重复序列（IRs，各26565 bp）隔开。叶绿体基因被成功注释，其中17个基因位于每个IR区域。厚朴叶绿体基因组特征与其他木兰类叶绿体基因组特征几乎相同。基于5个近缘物种的9个木兰样本共81个共享编码基因进行了系统发育分析。结果表明，在物种和种群水平上，区分物种通常比较简单。本研究证实了我们叶绿体基因组测序流程的有效性。叶绿体基因组可以提供鉴别差异，以帮助鉴定厚朴及其近缘植物。
