Bitner Sara E
Allegheny County Office of the Medical Examiner Forensic Laboratory, Forensic Biology Section, Pittsburgh, PA 15222, USA.
J Forensic Sci. 2012 Nov;57(6):1545-8. doi: 10.1111/j.1556-4029.2012.02141.x. Epub 2012 Apr 11.
In the course of the validation of a new component of the prostate-specific antigen (PSA) SemiQuant Cassette Test marketed by Seratec(®) , a false-positive reaction was observed when testing samples collected from the surface of unused, lubricated condoms. A variety of personal lubricants and condoms were tested to determine the frequency of the false positive, as well as its potential source. Samples were extracted in both water and the manufacturer-provided buffer, and the test was performed according to the manufacturer's suggested protocol. The false positive was observed intermittently, but occurred consistently with samples containing nonoxynol-9, a strong detergent utilized as a spermicide. The reaction may be attributable to the combination of latex and nonoxynol-9. Because of the unreliability of the test to confirm the presence of PSA in samples collected from condoms, the PSA cassette is an unsuitable method for confirming the presence of seminal fluid in condoms.
在对Seratec（®）销售的前列腺特异性抗原（PSA）半定量检测试剂盒的一种新组件进行验证的过程中，对从未使用过的、涂有润滑剂的避孕套表面采集的样本进行检测时，观察到了假阳性反应。对多种个人润滑剂和避孕套进行了测试，以确定假阳性的频率及其潜在来源。样本在水和制造商提供的缓冲液中进行提取，并按照制造商建议的方案进行检测。假阳性是间歇性观察到的，但在含有壬苯醇醚-9（一种用作杀精剂的强力去污剂）的样本中始终出现。该反应可能归因于乳胶和壬苯醇醚-9的组合。由于该检测方法无法可靠地确认从避孕套采集的样本中是否存在PSA，因此PSA检测试剂盒不是确认避孕套中是否存在精液的合适方法。
