State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, Jiangsu 210009, China.
Microbiol Res. 2012 Dec 20;167(10):616-22. doi: 10.1016/j.micres.2012.02.008. Epub 2012 Apr 10.
A marine actinomycete, designated strain BM-17, was isolated from a sediment sample collected in the Arctic Ocean. The strain was identified as Nocardia dassonvillei based on morphological, cultural, physiological, biochemical characteristics, along with the cell wall analysis and 16S rDNA gene sequence analysis. A new secondary metabolite (1), N-(2-hydroxyphenyl)-2-phenazinamine (NHP), and six known antibiotics (2-7) have been isolated from the saline culture broth of the stain by sequentially purification over macroporous resin D101, silica gel, Sephadex LH-20 column chromatography and preparative HPLC after the stain was incubated in soy bean media at 28°C for 7 days. The chemical structures of the compounds were elucidated on the basis of spectroscopic analysis, including two-dimensional (2D) NMR and HR-ESI-MS data. The new compound showed significant antifungal activity against Candida albicans, with a MIC of 64 μg/ml and high cancer cell cytotoxicity against HepG2, A549, HCT-116 and COC1 cells.
一株海洋放线菌，命名为菌株 BM-17，从北冰洋采集的沉积物样本中分离得到。该菌株基于形态学、培养特性、生理生化特性以及细胞壁分析和 16S rDNA 基因序列分析，被鉴定为达松维尔氏菌（Nocardia dassonvillei）。通过在大豆培养基中于 28°C 培养 7 天后，菌株在盐发酵液中依次用大孔树脂 D101、硅胶、Sephadex LH-20 柱色谱和制备型 HPLC 进行分离，得到了一个新的次生代谢产物（1），N-(2-羟基苯基)-2-吩嗪胺（NHP），以及六个已知抗生素（2-7）。化合物的结构是基于光谱分析，包括二维（2D）NMR 和 HR-ESI-MS 数据确定的。该新化合物对白色念珠菌表现出显著的抗真菌活性，MIC 为 64μg/ml，对 HepG2、A549、HCT-116 和 COC1 细胞具有较高的癌细胞细胞毒性。
