Song Yue, Li Qi-Yang, Cong Meng-Jing, Pang Xiao-Yan, Chen Bo, Liu Yong-Hong, Liao Li, Wang Jun-Feng
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine, Materia Medica/Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.
University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
Nat Prod Bioprospect. 2023 Oct 18;13(1):41. doi: 10.1007/s13659-023-00408-w.
Microbes well-adapted to the Arctic Ocean are promising for producing novel compounds, due to their fancy strategies for adaptation and being under-investigated. Two new phenazine alkaloids (1 and 2) and one new phenoxazine (3) were isolated from Nocardiopsis dassonvillei 502F, a strain originally isolated from Arctic deep-sea sediments. AntiSMASH analysis of the genome of Nocardiopsis dassonvillei 502F revealed the presence of 16 putative biosynthetic gene clusters (BGCs), including a phenazine BGC. Most of the isolated compounds were evaluated for their antibacterial, antiallergic, and cytotoxic activities. Among them, compounds 4 and 5 exhibited potent in vitro cytotoxic activities against osteosarcoma cell line 143B with IC values 0.16 and 20.0 μM, respectively. Besides, the results of antiallergic activities of compounds 6-8 exhibited inhibitory activities with IC values of 10.88 ± 3.05, 38.88 ± 3.29, and 2.44 ± 0.17 μg/mL, respectively (IC 91.6 μM for the positive control loratadine).
由于其独特的适应策略以及研究不足，适应北冰洋环境的微生物在生产新型化合物方面具有潜力。从最初从北极深海沉积物中分离出的达松维尔诺卡氏菌502F中分离出两种新的吩嗪生物碱（1和2）和一种新的吩恶嗪（3）。对达松维尔诺卡氏菌502F基因组的抗SMASH分析表明存在16个推定的生物合成基因簇（BGC），包括一个吩嗪BGC。对大多数分离出的化合物进行了抗菌、抗过敏和细胞毒性活性评估。其中，化合物4和5对骨肉瘤细胞系143B表现出强大的体外细胞毒性活性，IC值分别为0.16和20.0 μM。此外，化合物6 - 8的抗过敏活性结果显示出抑制活性，IC值分别为10.88±3.05、38.88±3.29和2.44±0.17 μg/mL（阳性对照氯雷他定的IC为91.6 μM）。
