胚胎多聚（A）结合蛋白（ePAB）磷酸化对于爪蟾卵母细胞成熟是必需的。
Embryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation.
机构信息
Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, 310 Cedar Street, LSOG 304D, New Haven, CT 06520, USA.
出版信息
Biochem J. 2012 Jul 1;445(1):93-100. doi: 10.1042/BJ20120304.
Abstract
Oocyte maturation and early embryonic development require the cytoplasmic polyadenylation and concomitant translational activation of stored maternal mRNAs. ePAB [embryonic poly(A)-binding protein, also known as ePABP and PABPc1-like] is a multifunctional post-transcriptional regulator that binds to poly(A) tails. In the present study we find that ePAB is a dynamically modified phosphoprotein in Xenopus laevis oocytes and show by mutation that phosphorylation at a four residue cluster is required for oocyte maturation. We further demonstrate that these phosphorylations are critical for cytoplasmic polyadenylation, but not for ePAB's inherent ability to promote translation. Our results provide the first insight into the role of post-translational modifications in regulating PABP protein activity in vivo.
摘要
卵母细胞成熟和早期胚胎发育需要细胞质聚腺苷酸化和储存的母体 mRNA 的翻译激活。ePAB（胚胎多聚腺苷酸化结合蛋白，也称为 ePABP 和 PABPc1 样）是一种多功能的转录后调控因子，可与多聚腺苷酸尾巴结合。在本研究中，我们发现 ePAB 是非洲爪蟾卵母细胞中一种动态修饰的磷酸蛋白，并通过突变表明，在四个残基簇上的磷酸化对于卵母细胞成熟是必需的。我们进一步证明，这些磷酸化对于细胞质聚腺苷酸化至关重要，但对于 ePAB 促进翻译的固有能力并非如此。我们的结果首次提供了关于翻译后修饰在体内调节 PABP 蛋白活性的作用的见解。
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