Animal Breeding and Genetics, Department of Animal Production, School of Agricultural Technology, Alexander Technological Educational Institute of Thessaloniki, Sindos, 57400, Greece.
Anim Genet. 2012 Dec;43(6):753-9. doi: 10.1111/j.1365-2052.2012.02346.x. Epub 2012 Mar 23.
Gilthead sea bream (Sparus aurata L.) is an important marine fish in Mediterranean aquaculture. Sex determination by age and/or body weight is a critical life-history trait, the genetic basis for which is largely unknown in this sequential hermaphrodite species. Herein, we performed a partial genome scan to map quantitative trait loci (QTL) affecting body weight and sex using 74 informative microsatellite markers from 10 paternal half-sib families to construct nine linkage groups (LG). In total, four growth-related QTL (two chromosome-wide and two genome-wide) and six QTL related to sex determination (three pairs in three different LGs) were detected (two chromosome-wide and one genome-wide). The proportion of phenotypic variation explained by the body-weight QTL ranged from 9.3% to 17.2%, showing their potential for use in marker-assisted selection. The results obtained offer solid ground to investigate the structure and function of the genomic regions involved in the mechanisms of sex reversal.
真鲷（Sparus aurata L.）是地中海水产养殖中的一种重要海水鱼。通过年龄和/或体重来确定性别是一个关键的生活史特征，而这种连续雌雄同体物种的性别决定的遗传基础在很大程度上尚不清楚。在此，我们利用 10 个父本半同胞家系中的 74 个信息丰富的微卫星标记进行部分基因组扫描，以构建 9 个连锁群（LG），来定位影响体重和性别的数量性状基因座（QTL）。共检测到 4 个与生长相关的 QTL（2 个染色体-wide 和 2 个全基因组）和 6 个与性别决定相关的 QTL（3 对在 3 个不同的 LG 中）（2 个染色体-wide 和 1 个全基因组）。体重 QTL 解释的表型变异比例范围为 9.3%至 17.2%，表明它们具有在标记辅助选择中应用的潜力。所获得的结果为研究参与性反转机制的基因组区域的结构和功能提供了坚实的基础。
