Department of Plant and Animal Sciences, Nova Scotia Agricultural College, Truro, Nova Scotia, B2N 5E3, Canada.
Prev Vet Med. 2012 Oct 1;106(3-4):332-8. doi: 10.1016/j.prevetmed.2012.03.010. Epub 2012 Apr 11.
Despite many years of testing mink for serum antibodies against the Aleutian mink disease virus (AMDV) by counterimmunoelectrophoresis (CIEP) and elimination of reactors, this virus has remained the number one disease threat for the mink industry in Nova Scotia (NS). The objective of this study was to analyze CIEP test results to determine the success of the AMDV-control strategy in NS. A total of 2,964,920 CIEP test results from 82 ranches, spanning an eight-year period between 1998 and 2005, were analyzed. This survey included approximately 60% of the active ranchers in the province. The number of ranchers that tested their animals was 42 in 1998, gradually increased to 58 in 2003 and then showed some decline. The overall proportion of CIEP-positive mink was 3.34%, and varied between 5.22% in 1999 and 1.35% in 2005. The proportion of infected ranches ranged between 23.8% in 1998 and 70.7% in 2003. The overall trend was for a smaller proportion of infected animals but a larger proportion of infected ranches during this time period. Of the 82 ranches, 24 (29.3%) had negative CIEP in all tests, 15 (18.3%) had CIEP positive animals in every year tested, and 43 (52.4%) had positive and negative results in different years, indicating that AMDV infection was widespread in NS. There were 23 infected ranches with 8 years of uninterrupted testing. These ranchers performed 75.8% of the total samples tested (2,246,711), implying that they have diligently been trying to eradicate the virus. Infection persisted on three of these ranches for the entire 8 year period, and only two of the ranches remained CIEP negative for longer than four years. The average percentage of CIEP-positive mink on these ranches was 2.2, which was lower than 6.35% for the 33 infected ranches with occasional testing, and 73.6% and 82.4% for two ranches that had never used the CIEP test, showing that persistent test-and-removal strategy has been effective in reducing the prevalence of infected animals but has failed to eradicate the virus from most of the infected ranches.
尽管新斯科舍省（NS）多年来一直通过对流免疫电泳（CIEP）检测水貂血清中的抗阿留申病病毒（AMDV）抗体，并淘汰反应者，但该病毒仍然是水貂业面临的头号疾病威胁。本研究的目的是分析 CIEP 检测结果，以确定 NS 中 AMDV 控制策略的成功。对 1998 年至 2005 年 8 年间 82 个牧场的 2964920 次 CIEP 检测结果进行了分析。该调查包括该省约 60%的活跃牧场主。1998 年，进行动物检测的牧场主有 42 个，2003 年逐渐增加到 58 个，然后有所下降。CIEP 阳性水貂的总体比例为 3.34%，1999 年为 5.22%，2005 年为 1.35%。感染牧场的比例在 1998 年为 23.8%，在 2003 年为 70.7%。总体趋势是在此期间，感染动物的比例较小，但感染牧场的比例较大。在 82 个牧场中，24 个（29.3%）在所有检测中 CIEP 均为阴性，15 个（18.3%）在每年的检测中 CIEP 阳性动物，43 个（52.4%）在不同年份的检测中 CIEP 阳性和阴性结果，表明 AMDV 感染在 NS 广泛存在。有 23 个感染牧场连续 8 年不间断检测。这些牧场主进行了总样本测试的 75.8%（2246711 个），这意味着他们一直在努力根除病毒。在整个 8 年期间，有 3 个牧场的感染持续存在，只有 2 个牧场的 CIEP 阴性持续超过 4 年。这些牧场的 CIEP 阳性水貂平均比例为 2.2%，低于偶尔进行检测的 33 个感染牧场的 6.35%，也低于从未使用 CIEP 检测的两个牧场的 73.6%和 82.4%，表明持续的检测和淘汰策略已有效降低了感染动物的流行率，但未能从大多数感染牧场根除病毒。
