Farid A Hossain, Ferns Linda E
Department of Animal Science and Aquaculture, Faculty of Agriculture, Dalhousie University, Canada.
Pathology Laboratory, Veterinary Services, Nova Scotia Department of Agriculture, Canada.
Res Vet Sci. 2017 Apr;111:127-134. doi: 10.1016/j.rvsc.2017.02.009. Epub 2017 Feb 16.
The objective of this study was to measure the effect of selection for tolerance on the severity of the Aleutian disease (AD) lesions in mink. Sensitivity and specificity of antibody detection in the blood by counter-immunoelectrophoresis (CIEP) relative to the presence of Aleutian mink disease virus (AMDV) in the spleen by PCR in naturally infected farmed mink were also estimated. Carcasses of 680 sero-positive (CIEP-P) black mink from 28 farms in Nova Scotia, Canada, and from 132 sero-negative (CIEP-N) mink from 14 of these farms were collected at pelting time. A total of 116 of the CIEP-P mink were from three farms where animals have been selected for tolerating AD for almost 20years. The severity of the AD lesions was assessed by histopathological examination of kidneys, lungs, heart, brain and liver on a scale of 0 to 4. Sensitivity and specificity of CIEP relative to PCR were 0.97 and 0.85, respectively, and 16.5% of CIEP-N mink were PCR positive, which could be one of the reasons for the failure of virus eradication by CIEP in Canada. The CIEP-N and tolerant CIEP-P animals had 9.39 and 6.23 greater odds of showing lower lesion severity, respectively, than the CIEP-P animals (P<0.01). The CIEP-N mink had a slightly higher chance (P=0.07) of showing lower lesion severity (odds ratio 1.51) compared with tolerant CIEP-P mink. The results suggested that tolerant mink had significantly reduced severity of AD lesions despite having anti-viral antibodies and carrying the virus.
本研究的目的是测定选择耐受性对水貂阿留申病（AD）病变严重程度的影响。还评估了在自然感染的养殖水貂中，通过对流免疫电泳（CIEP）检测血液中抗体相对于通过聚合酶链反应（PCR）检测脾脏中是否存在阿留申水貂病病毒（AMDV）的敏感性和特异性。在加拿大新斯科舍省28个养殖场的680只血清阳性（CIEP-P）黑色水貂以及其中14个养殖场的132只血清阴性（CIEP-N）水貂在取皮时被收集。共有116只CIEP-P水貂来自三个养殖场，在这些养殖场中，动物被选择耐受AD病已近20年。通过对肾脏、肺、心脏、大脑和肝脏进行组织病理学检查，以0至4级评估AD病变的严重程度。CIEP相对于PCR的敏感性和特异性分别为0.97和0.85，16.5%的CIEP-N水貂PCR呈阳性，这可能是加拿大通过CIEP根除病毒失败的原因之一。CIEP-N和耐受性CIEP-P动物显示较低病变严重程度的几率分别比CIEP-P动物高9.39和6.23倍（P<0.01）。与耐受性CIEP-P水貂相比，CIEP-N水貂显示较低病变严重程度的几率略高（P=0.07）（优势比为1.51）。结果表明，尽管耐受性水貂具有抗病毒抗体并携带病毒，但其AD病变的严重程度显著降低。
