Institute of Molecular Systems Biology, ETH Zürich, Wolfgang-Pauli-Strasse 16, Zürich, Switzerland.
J Proteomics. 2012 Jun 18;75(11):3211-20. doi: 10.1016/j.jprot.2012.03.030. Epub 2012 Apr 3.
Parvalbumins beta (β-PRVBs) are considered the major fish allergens. A new strategy for the rapid and direct detection of these allergens in any foodstuff is presented in this work. The proposed methodology is based on the purification of β-PRVBs by treatment with heat, the use of accelerated in-solution trypsin digestion under an ultrasonic field provided by High-Intensity Focused Ultrasound (HIFU) and the monitoring of only nineteen β-PRVB peptide biomarkers by Selected MS/MS Ion Monitoring (SMIM) in a linear ion trap (LIT) mass spectrometer. The present strategy allows the direct detection of the presence of fish β-PRVBs in any food product in less than 2 hours.
副肌球蛋白β（β-PRVBs）被认为是主要的鱼类过敏原。本工作提出了一种在任何食品中快速直接检测这些过敏原的新策略。该方法基于通过热处理纯化β-PRVBs，在高强度聚焦超声（HIFU）提供的超声场下使用加速溶液内胰蛋白酶消化，以及仅通过线性离子阱（LIT）质谱仪中的选择 MS/MS 离子监测（SMIM）监测十九个 β-PRVB 肽生物标志物。本策略允许在不到 2 小时的时间内直接检测任何食品产品中是否存在鱼类β-PRVBs。
