Ha Yeonjeong
ICT Environment Convergence, Department of ICT Convergence, College of IT Engineering, Pyeongtaek University, 3825 Seodong-daero, Pyeongtaek-si 17869, Gyeonggi-do, Republic of Korea.
Diagnostics (Basel). 2023 Sep 23;13(19):3033. doi: 10.3390/diagnostics13193033.
Magnetic nanoparticles (MNPs) have emerged as a promising material in disease diagnostics due to their potential to enhance detection sensitivity, facilitate concentration and purification of target substances in diverse samples, and enable favorable color-based detection. In this study, antibody-conjugated MNPs were successfully synthesized and validated through two appropriate methods: the measurement of MNPs' size and the use of phosphatase methods. Additionally, three methods were suggested and implemented for developing color in MNPs-based immunoassay, including the formation of MNP aggregations, utilization of MNPs' peroxidase-like activity, and synthesis of dually-conjugated MNPs with both enzyme and antibody. In particular, color development utilizing nanoparticle aggregations was demonstrated to result in a more yellowish color as virus concentration increased, while the peroxidase activity of MNPs exhibited a proportional increase in color intensity as the MNP concentration increased. This observation suggests the potential applicability of quantitative analysis using these methods. Furthermore, effective concentration and purification of target substances were demonstrated through the collection of MNPs using an external magnetic field, irrespective of factors such as antibody conjugation, dispersion medium, or virus binding. Finally, based on the key findings of this study, a design proposal for MNPs-based immunoassay is presented. Overall, MNPs-based immunoassays hold significant potential for advancing disease diagnostics.
磁性纳米颗粒（MNPs）因其具有提高检测灵敏度、促进不同样品中目标物质的浓缩和纯化以及实现基于颜色的良好检测的潜力，已成为疾病诊断中有前景的材料。在本研究中，通过两种合适的方法成功合成并验证了抗体偶联的MNPs：测量MNPs的大小和使用磷酸酶方法。此外，还提出并实施了三种在基于MNPs的免疫测定中显色的方法，包括形成MNP聚集体、利用MNPs的过氧化物酶样活性以及合成同时偶联酶和抗体的双偶联MNPs。特别地，利用纳米颗粒聚集体显色被证明随着病毒浓度增加颜色更偏黄，而MNPs的过氧化物酶活性随着MNP浓度增加颜色强度呈比例增加。这一观察结果表明使用这些方法进行定量分析的潜在适用性。此外，通过使用外部磁场收集MNPs证明了目标物质的有效浓缩和纯化，而与抗体偶联、分散介质或病毒结合等因素无关。最后，基于本研究的关键发现，提出了基于MNPs的免疫测定的设计方案。总体而言，基于MNPs的免疫测定在推进疾病诊断方面具有巨大潜力。
