State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China.
J Agric Food Chem. 2012 May 2;60(17):4276-81. doi: 10.1021/jf205056u. Epub 2012 Apr 18.
A polysaccharide (GSP-6B) with a molecular mass of 1.86 × 10⁶ Da was isolated from the fruiting bodies of Ganoderma sinense . Chemical composition analysis, methylation analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were conducted to elucidate its structure. GSP-6B contains a backbone of (1→6)-linked-β-D-glucopyranosyl residues, bearing branches at the O-3 position of every two sugar residues along the backbone. The side chains contain (1→4)-linked-β-D-glucopyranosyl residues, (1→3)-linked-β-D-glucopyranosyl residues, and nonreducing end β-D-glucopyranosyl residues. An in vitro immunomodulating activity assay revealed that GSP-6B could significantly induce the release of IL-1β and TNF-α in human peripheral blood mononuclear cell (PBMC) and showed no toxicity to either PBMC or a human macrophage cell line THP-1. GSP-6B could also activate dendritic cells (DC) by stimulating the secretion of IL-12 and IL-10 from DC.
从灵芝的子实体中分离得到一种分子量为 1.86×106 Da 的多糖（GSP-6B）。通过化学组成分析、甲基化分析、红外光谱和核磁共振波谱对其结构进行了阐明。GSP-6B 的主链由（1→6）-连接的-β-D-吡喃葡萄糖基残基组成，在主链上每隔两个糖基的 O-3 位带有支链。侧链含有（1→4）-连接的-β-D-吡喃葡萄糖基残基、（1→3）-连接的-β-D-吡喃葡萄糖基残基和非还原端的β-D-吡喃葡萄糖基残基。体外免疫调节活性试验表明，GSP-6B 能显著诱导人外周血单个核细胞（PBMC）释放 IL-1β 和 TNF-α，对 PBMC 或人巨噬细胞系 THP-1 均无毒性。GSP-6B 还能通过刺激树突状细胞（DC）分泌 IL-12 和 IL-10 来激活 DC。
