Ramatla Tsepo, Mileng Kealeboga, Ndou Rendani, Mphuti Nthabiseng, Syakalima Michelo, Lekota Kgaugelo E, Thekisoe Oriel M M
Department of Animal Health, School of Agriculture, North-West University, Private Bag X2046, Mmabatho 2735, South Africa.
Unit for Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom 2531, South Africa.
Microorganisms. 2022 Jan 28;10(2):313. doi: 10.3390/microorganisms10020313.
The rapid growth of multidrug-resistant is a global public health concern. The aim of this study was to detect integrons, colistin and resistance genes in and . A total of 63 isolates of ( = 18) and ( = 45) from fecal samples of layers and rats at chicken farms were screened for antibiotic resistant genes. Conventional PCR was performed for the detection of integrons (classes 1, 2, and 3), colistin (-1-5) and β-lactamase (, , , , , , , and ) resistant genes. Of these isolates, 77% and 27% of and harboured the 4 encoded gene for colistin, respectively. The prevalence of class 1 integrons for and was 100% for each serovar, while for class 2 integrons of and it was 49% and 33% respectively, while class 3 integron genes was not detected. Our study also detected high levels of encoding genes ( gene), namely , , and from both and . This, to our knowledge, is the first report of resistance gene detection in serovars in South Africa. This study also highlights the importance of controlling rats at poultry farms in order to reduce the risk of transmission of antibiotic resistance to chickens and eventually to humans.
多重耐药菌的快速增长是一个全球公共卫生问题。本研究的目的是检测鸡和大鼠中的整合子、黏菌素及耐药基因。对来自养鸡场蛋鸡和大鼠粪便样本中的63株鸡源大肠杆菌（n = 18）和鼠源大肠杆菌（n = 45）进行了抗生素耐药基因筛查。采用常规PCR检测整合子（1、2和3类）、黏菌素（mcr-1-5）和β-内酰胺酶（blaTEM、blaSHV、blaCTX-M、blaOXA-1、blaOXA-2、blaOXA-10、blaOXA-23和blaOXA-48）耐药基因。在这些分离株中，鸡源大肠杆菌和鼠源大肠杆菌分别有77%和27%携带黏菌素的mcr-4编码基因。每个血清型的鸡源大肠杆菌和鼠源大肠杆菌中1类整合子的流行率均为100%，而2类整合子在鸡源大肠杆菌和鼠源大肠杆菌中的流行率分别为49%和33%，未检测到3类整合子基因。我们的研究还从鸡源大肠杆菌和鼠源大肠杆菌中检测到了高水平的blaCTX-M编码基因（CTX-M基因），即blaCTX-M-1、blaCTX-M-2、blaCTX-M-9和blaCTX-M-15。据我们所知，这是南非首次报道鸡血清型中耐药基因的检测。本研究还强调了控制家禽场老鼠的重要性，以降低抗生素耐药性传播给鸡并最终传播给人类的风险。
