Wright P A, Wynford-Thomas D
Department of Pathology, University of Wales College of Medicine, Cardiff, U.K.
J Pathol. 1990 Oct;162(2):99-117. doi: 10.1002/path.1711620203.
Since publication of the polymerase chain reaction (PCR) technique in 1985 (Saiki et al. Science 1985; 230: 1350-1354), there has been an explosion of reports on its use in medicine and science. We critically review its use both as a diagnostic technique and as a research tool, and show the pathologist how to evaluate PCR data and how to avoid the pitfalls of overinterpretation. We discuss the value of PCR in the characterization of genetic defects, prenatal diagnosis, carrier testing, HLA typing, detecting micro-organisms, identifying activated oncogenes, and in the characterization of leukaemias and lymphomas, and summarize the main applications in biomedical research.
自1985年聚合酶链反应（PCR）技术问世以来（Saiki等人，《科学》，1985年；230: 1350 - 1354），关于其在医学和科学领域应用的报道如雨后春笋般涌现。我们对其作为诊断技术和研究工具的应用进行了批判性回顾，并向病理学家展示如何评估PCR数据以及如何避免过度解读的陷阱。我们讨论了PCR在基因缺陷特征分析、产前诊断、携带者检测、HLA分型、微生物检测、活化癌基因鉴定以及白血病和淋巴瘤特征分析中的价值，并总结了其在生物医学研究中的主要应用。
