Department of Bioengineering and COMSET, Clemson University, Clemson, South Carolina 29634, USA.
Opt Lett. 2012 Mar 1;37(5):827-9. doi: 10.1364/OL.37.000827.
We developed a novel addressable discrete-line-scanning multiphoton microscope with high lateral and axial resolutions based on a spatial light modulator. Our discrete-line-focus design eliminates the cross talk that occurs in conventional one-dimensional line-scanning multiphoton microscopies. Additionally, a phase-only spatial light modulator is able to scan only a sample's target area by generating a specific discrete line focus according to the shape and location of the target area. Compared with other multiphoton microscopies, this technique shortens scanning time and minimizes photodamage by concentrating scanning energy and dwell time on the area of interest.
我们基于空间光调制器开发了一种具有高横向和轴向分辨率的新型寻址离散线扫描多光子显微镜。我们的离散线焦点设计消除了传统一维线扫描多光子显微镜中发生的串扰。此外，仅相位空间光调制器能够通过根据目标区域的形状和位置生成特定的离散线焦点来仅扫描样品的目标区域。与其他多光子显微镜相比，该技术通过将扫描能量和驻留时间集中在感兴趣的区域上，缩短了扫描时间并最小化了光损伤。
