Water and Soil Protection Laboratory, Environmental Organic Chemistry Group, Office of Water and Waste, Schermenweg 11, Bern, Switzerland.
Chemosphere. 2012 Jul;88(5):563-9. doi: 10.1016/j.chemosphere.2012.03.030. Epub 2012 Apr 12.
A HPLC-MS/MS method is presented for the simultaneous determination of frequently used artificial sweeteners (ASs) and the main metabolite of aspartame (ASP), diketopiperazine (DKP), in environmental water samples using the direct-injection (DI) technique, thereby achieving limits of quantification (LOQ) of 10 ng L(-1). For a reliable quantification of ASP pH should be adjusted to 4.3 to prevent formation of the metabolite. Acesulfame (ACE), saccharin (SAC), cyclamate (CYC) and sucralose (SUC) were ubiquitously found in water samples. Highest concentrations up to 61 μg L(-1) of ACE were found in wastewater effluents, followed by surface water with concentrations up to 7 μg L(-1), lakes up to 600 ng L(-1) and groundwater and tap water up to 70 ng L(-1). The metabolite DKP was only detected in wastewater up to 200 ng L(-1) and at low detection frequencies.
本文提出了一种高效液相色谱-串联质谱法（HPLC-MS/MS），用于直接进样（DI）技术同时测定环境水样中常用人工甜味剂（ASs）和阿斯巴甜（ASP）的主要代谢物二酮哌嗪（DKP）。该方法的定量下限（LOQ）为 10 ng L(-1)。为了可靠地定量 ASP，应将 pH 值调节至 4.3，以防止代谢物的形成。在水样中普遍检测到乙酰磺胺酸钾（ACE）、糖精（SAC）、环已基氨基磺酸钠（CYC）和三氯蔗糖（SUC）。在废水废水中发现 ACE 的最高浓度高达 61 μg L(-1)，其次是地表水，浓度高达 7 μg L(-1)，湖泊高达 600 ng L(-1)，地下水和自来水高达 70 ng L(-1)。代谢物 DKP 仅在废水废水中检测到低浓度（最高 200 ng L(-1)），且检测频率较低。
