Santa T, Al-Dirbashi O Y, Fukushima T
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
Drug Discov Ther. 2007 Oct;1(2):108-18.
Liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESIMS/MS) is one of the most prominent analytical techniques owing to its inherent selectivity and sensitivity. In LC/ESI-MS/MS, chemical derivatization is frequently used to enhance the MS/MS detectability. The derivatization improves the separation and ionization efficiency. Moreover, the generated derivatives give particular product ions by CID (collision induced dissociation), which allow for the sensitive detection. In this review, we present an overview of the derivatization reagents which have been applied to LC/ESI-MS/MS, focusing on the applications involving small molecules in biomatrices.
液相色谱/电喷雾电离串联质谱法（LC/ESIMS/MS）因其固有的选择性和灵敏度，成为最突出的分析技术之一。在LC/ESI-MS/MS中，化学衍生化常用于提高MS/MS的检测能力。衍生化可提高分离和电离效率。此外，生成的衍生物通过碰撞诱导解离（CID）产生特定的产物离子，从而实现灵敏检测。在本综述中，我们概述了已应用于LC/ESI-MS/MS的衍生化试剂，重点关注涉及生物基质中小分子的应用。
