Shao Jing, Guo Mei, Fan Qin, Cheng Fang
Gansu University of Traditional Medicine, Lanzhou 730000, China.
Zhong Yao Cai. 2011 Nov;34(11):1678-81.
To establish the quantitative determination method and TLC identification of Meconopsis and study the Vis-UV spectrum character for fast identification.
Meconopsis were identified by TLC, and the content of quercetin was determined by HPLC.
The acid hydrolyze condition was HCl-80% MeOH(8:25) 90 degrees C calefaction by water for 1.5 hour. The condition of HPLC: Hypersil-Keystone-C18 (250 mm x 4.6 mm, 5 microm) column, MeOH-Water (50: 50) as mobile phase, detection wavelength at 360nm, flow rate at 1.0 mL/min, column temperature at 35 degrees C, quercetin showed a good linear relationship at a range of 0.01 - 0.12 microg, r =0.9997. The average recovery was 98.83%, and RSD was 1.69% (n = 9). The contents of quercetin in Meconopsis integrifolia and Meconopsis quintuplinervia of Gansu were 0.1135 mg/g and 0.0842 mg/g respectively.
The method is simple, accurate and specific. It can be used for the quality control and fast identification of Meconopsis.
建立绿绒蒿属植物的定量测定方法和薄层色谱鉴别方法，并研究其可见-紫外光谱特征以进行快速鉴别。
采用薄层色谱法对绿绒蒿属植物进行鉴别，采用高效液相色谱法测定槲皮素含量。
酸水解条件为盐酸-80%甲醇（8:25），90℃水浴加热1.5小时。高效液相色谱条件：Hypersil-Keystone-C18（250mm×4.6mm，5μm）色谱柱，甲醇-水（50:50）为流动相，检测波长360nm，流速1.0mL/min，柱温35℃，槲皮素在0.01 - 0.12μg范围内呈良好线性关系，r = 0.9997。平均回收率为98.83%，相对标准偏差为1.69%（n = 9）。甘肃全缘叶绿绒蒿和五脉绿绒蒿中槲皮素含量分别为0.1135mg/g和0.0842mg/g。
该方法简便、准确、专属性强。可用于绿绒蒿属植物的质量控制和快速鉴别。
