Shi Xia, Cheng Fang, Guo Mei, Shao Jing
Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China.
Zhongguo Zhong Yao Za Zhi. 2011 Dec;36(23):3290-2.
To develop an HPLC method for the determination of a Tibetan medicine Meconopsis quintuplinervia.
A Hypersil-Keystone-C18 column (4.6 mm x 250 mm, 5 microm) was used with the isocratic elution of acetonitrile and 0.012% glacial acetic acid. The flow rate was 1.0 mL x min(-1), and the detection wavelength was set at 237 nm.
The linear range of 0-methylflavinantine was 0.2-2.4 microg (r = 0.999 7). The average recovery was 96.26%.
The developed method was reliable, and can be used for the quality control of M. quintuplinervia Regel.
建立一种测定藏药五脉绿绒蒿的高效液相色谱法。
采用Hypersil-Keystone-C18柱（4.6 mm×250 mm，5μm），以乙腈和0.012%冰醋酸等度洗脱。流速为1.0 mL·min⁻¹，检测波长设定为237 nm。
0-甲基黄罂粟碱的线性范围为0.2 - 2.4μg（r = 0.999 7）。平均回收率为96.26%。
所建立的方法可靠，可用于五脉绿绒蒿的质量控制。
