State Research Center for Applied Microbiology & Biotechnology, Obolensk, Russia.
FEMS Microbiol Lett. 2012 Jul;332(1):40-6. doi: 10.1111/j.1574-6968.2012.02573.x. Epub 2012 May 8.
Acinetobacter baumannii plays a significant role in infecting patients admitted to hospitals. Many A. baumannii infections, including ventilation-associated pneumonia, wound, and bloodstream infections, are common for intensive care and burn units. The ability of the microorganism to acquire resistance to many antibiotics, disinfectants, and dehydration assures its long-term survival in hospital settings. The application of bacteriophages is a potential tool to control A. baumannii infections. Bacteriophage AP22 lytic for A. baumannii was isolated from clinical materials and classified as a member of the Myoviridae family. The phage had an icosahedral head of 64 nm in diameter and a contractile tail of 85-90 nm in length. According to restriction analysis, AP22 had 46-kb double-stranded DNA genome. The phage AP22 exhibited rapid adsorption (> 99% adsorbed in 5 min), a large burst size (240 PFU per cell), and stability to the wide range of pH. The bacteriophage was shown to specifically infect and lyse 68% (89 of 130) genotype-varying multidrug-resistant clinical A. baumannii strains by forming clear zones. Thus, it could be used as a candidate for making up phage cocktails to control A. baumannii-associated nosocomial infections.
鲍曼不动杆菌在感染住院患者方面起着重要作用。许多鲍曼不动杆菌感染，包括呼吸机相关性肺炎、伤口和血流感染，在重症监护和烧伤病房很常见。该微生物能够获得对许多抗生素、消毒剂和脱水剂的耐药性，确保其在医院环境中的长期生存。噬菌体的应用是控制鲍曼不动杆菌感染的一种潜在工具。从临床标本中分离出一株对鲍曼不动杆菌具有裂解作用的噬菌体 AP22，并将其归类为肌尾噬菌体科的成员。噬菌体有一个直径为 64nm 的二十面体头部和一个 85-90nm 长的收缩尾。根据限制分析，AP22 有一个 46kb 的双链 DNA 基因组。噬菌体 AP22 表现出快速吸附（在 5 分钟内吸附率超过 99%）、大爆发量（每个细胞产生 240 个 PFU）和对广泛 pH 值的稳定性。噬菌体被证明能够特异性感染和裂解 68%（130 株基因型多样的多药耐药临床鲍曼不动杆菌中有 89 株）的菌株，形成清晰的噬菌斑。因此，它可以作为制备噬菌体鸡尾酒以控制与鲍曼不动杆菌相关的医院获得性感染的候选物。
