• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

正电子发射断层扫描报告基因和报告探针：基因和细胞治疗应用。

Positron emission tomography reporter genes and reporter probes: gene and cell therapy applications.

机构信息

1. UCLA School of Medicine, Department of Molecular and Medical Pharmacology.

出版信息

Theranostics. 2012;2(4):374-91. doi: 10.7150/thno.3677. Epub 2012 Apr 10.

DOI:10.7150/thno.3677
PMID:22509201
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3326723/
Abstract

Positron emission tomography (PET) imaging reporter genes (IRGs) and PET reporter probes (PRPs) are amongst the most valuable tools for gene and cell therapy. PET IRGs/PRPs can be used to non-invasively monitor all aspects of the kinetics of therapeutic transgenes and cells in all types of living mammals. This technology is generalizable and can allow long-term kinetics monitoring. In gene therapy, PET IRGs/PRPs can be used for whole-body imaging of therapeutic transgene expression, monitoring variations in the magnitude of transgene expression over time. In cell or cellular gene therapy, PET IRGs/PRPs can be used for whole-body monitoring of therapeutic cell locations, quantity at all locations, survival and proliferation over time and also possibly changes in characteristics or function over time. In this review, we have classified PET IRGs/PRPs into two groups based on the source from which they were derived: human or non-human. This classification addresses the important concern of potential immunogenicity in humans, which is important for expansion of PET IRG imaging in clinical trials. We have then discussed the application of this technology in gene/cell therapy and described its use in these fields, including a summary of using PET IRGs/PRPs in gene and cell therapy clinical trials. This review concludes with a discussion of the future direction of PET IRGs/PRPs and recommends cell and gene therapists collaborate with molecular imaging experts early in their investigations to choose a PET IRG/PRP system suitable for progression into clinical trials.

摘要

正电子发射断层扫描（PET）成像报告基因（IRG）和 PET 报告探针（PRP）是基因和细胞治疗中最有价值的工具之一。PET IRG/PRP 可用于非侵入性地监测治疗性转基因和细胞在所有类型活体哺乳动物中的动力学的各个方面。该技术具有通用性，可以实现长期动力学监测。在基因治疗中，PET IRG/PRP 可用于治疗性转基因表达的全身成像，监测转基因表达随时间的变化。在细胞或细胞基因治疗中，PET IRG/PRP 可用于全身监测治疗细胞的位置、所有位置的数量、随时间的存活和增殖，以及随时间的特征或功能变化。在这篇综述中，我们根据其来源将 PET IRG/PRP 分为两类：人类或非人类。这种分类解决了人类潜在免疫原性的重要问题，这对于在临床试验中扩大 PET IRG 成像的应用非常重要。然后，我们讨论了该技术在基因/细胞治疗中的应用，并描述了它在这些领域的使用，包括总结了在基因和细胞治疗临床试验中使用 PET IRG/PRP 的情况。本文最后讨论了 PET IRG/PRP 的未来发展方向，并建议细胞和基因治疗师在其研究早期与分子成像专家合作，选择适合进入临床试验的 PET IRG/PRP 系统。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/e1db4df53885/thnov02p0374g12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/780a0d85b5b9/thnov02p0374g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/80584b16a4a7/thnov02p0374g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/c3ec47f18355/thnov02p0374g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/9007ba5d926a/thnov02p0374g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/aa27c2cd3da7/thnov02p0374g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/54894efa3446/thnov02p0374g08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/eab0d5f499eb/thnov02p0374g09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/fc214832fc7c/thnov02p0374g11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/e1db4df53885/thnov02p0374g12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/780a0d85b5b9/thnov02p0374g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/80584b16a4a7/thnov02p0374g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/c3ec47f18355/thnov02p0374g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/9007ba5d926a/thnov02p0374g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/aa27c2cd3da7/thnov02p0374g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/54894efa3446/thnov02p0374g08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/eab0d5f499eb/thnov02p0374g09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/fc214832fc7c/thnov02p0374g11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ad7/3326723/e1db4df53885/thnov02p0374g12.jpg

相似文献

1
Positron emission tomography reporter genes and reporter probes: gene and cell therapy applications.正电子发射断层扫描报告基因和报告探针：基因和细胞治疗应用。
Theranostics. 2012;2(4):374-91. doi: 10.7150/thno.3677. Epub 2012 Apr 10.
2
Recent advances in imaging endogenous or transferred gene expression utilizing radionuclide technologies in living subjects: applications to breast cancer.利用放射性核素技术在活体受试者中成像内源性或转移基因表达的最新进展：在乳腺癌中的应用
Breast Cancer Res. 2001;3(1):28-35. doi: 10.1186/bcr267. Epub 2000 Dec 11.
3
Comparison of the Efficacy and Sensitivity of Alternative PET Reporter Gene/PET Reporter Probe Systems That Minimize Biological Variables.比较不同的 PET 报告基因/PET 报告探针系统，以最小化生物学变量的功效和灵敏度。
Methods Mol Biol. 2020;2126:177-190. doi: 10.1007/978-1-0716-0364-2_16.
4
PET reporter genes for noninvasive imaging of gene therapy, cell tracking and transgenic analysis.用于基因治疗、细胞追踪和转基因分析无创成像的正电子发射断层显像（PET）报告基因。
Crit Rev Oncol Hematol. 2004 Sep;51(3):191-204. doi: 10.1016/j.critrevonc.2004.04.006.
5
Positron emission tomography reporter gene imaging in the myocardium: for monitoring of angiogenic gene therapy in ischemic heart disease.心肌中的正电子发射断层扫描报告基因成像：用于监测缺血性心脏病的血管生成基因治疗。
J Card Surg. 2005 Nov-Dec;20(6):S20-4. doi: 10.1111/j.1540-8191.2005.00152.x.
6
Seeing is believing: non-invasive, quantitative and repetitive imaging of reporter gene expression in living animals, using positron emission tomography.眼见为实：使用正电子发射断层扫描对活体动物体内报告基因表达进行非侵入性、定量和重复性成像。
J Neurosci Res. 2000 Mar 15;59(6):699-705. doi: 10.1002/(SICI)1097-4547(20000315)59:6<699::AID-JNR1>3.0.CO;2-1.
7
PET imaging for gene & cell therapy.正电子发射断层成像术用于基因和细胞治疗。
Curr Gene Ther. 2012 Feb 1;12(1):20-32. doi: 10.2174/156652312799789271.
8
Studying the biodistribution of positron emission tomography reporter probes in mice.研究正电子发射断层扫描报告探针在小鼠体内的生物分布。
Nat Protoc. 2007;2(7):1752-5. doi: 10.1038/nprot.2007.228.
9
Non-invasive imaging of cardiac transgene expression with PET: comparison of the human sodium/iodide symporter gene and HSV1-tk as the reporter gene.利用正电子发射断层扫描（PET）对心脏转基因表达进行无创成像：以人钠/碘同向转运体基因和单纯疱疹病毒1型胸苷激酶（HSV1-tk）作为报告基因的比较
Eur J Nucl Med Mol Imaging. 2005 Sep;32(9):1108-14. doi: 10.1007/s00259-005-1854-4.
10
Positron emission tomography: measurement of transgene expression.正电子发射断层扫描：转基因表达的测量。
Methods. 2002 Jul;27(3):234-41. doi: 10.1016/s1046-2023(02)00080-4.

引用本文的文献

1
Approaches to Imaging Immune Activation Using PET.利用正电子发射断层扫描（PET）对免疫激活进行成像的方法。
J Nucl Med. 2025 Jun 2;66(6):839-847. doi: 10.2967/jnumed.124.268289.
2
Recent advances in non-invasive tracking of cell-based cancer immunotherapies.基于细胞的癌症免疫疗法非侵入性追踪的最新进展。
Biomater Sci. 2025 Apr 8;13(8):1939-1959. doi: 10.1039/d4bm01677g.
3
Molecular imaging of viral pathogenesis and opportunities for the future.病毒发病机制的分子成像及未来机遇。

本文引用的文献

1
Structure-guided engineering of human thymidine kinase 2 as a positron emission tomography reporter gene for enhanced phosphorylation of non-natural thymidine analog reporter probe.基于结构的人胸苷激酶 2 工程改造作为正电子发射断层扫描报告基因，用于增强非天然胸苷类似物报告探针的磷酸化。
J Biol Chem. 2012 Jan 2;287(1):446-454. doi: 10.1074/jbc.M111.314666. Epub 2011 Nov 9.
2
A new pyrimidine-specific reporter gene: a mutated human deoxycytidine kinase suitable for PET during treatment with acycloguanosine-based cytotoxic drugs.一种新型嘧啶特异性报告基因：一种突变型人脱氧胞苷激酶，适用于基于阿昔洛韦的细胞毒性药物治疗期间的 PET。
J Nucl Med. 2010 Sep;51(9):1395-403. doi: 10.2967/jnumed.109.074344.
3
Npj Imaging. 2025;3(1):3. doi: 10.1038/s44303-024-00056-w. Epub 2025 Jan 24.
4
Development of an antigen-based approach to noninvasively image CAR T cells in real time and as a predictive tool.开发一种基于抗原的方法，实时无创性地成像 CAR T 细胞，并将其作为一种预测工具。
Sci Adv. 2024 Sep 20;10(38):eadn3816. doi: 10.1126/sciadv.adn3816. Epub 2024 Sep 18.
5
Imaging CAR-NK cells targeted to HER2 ovarian cancer with human sodium-iodide symporter-based positron emission tomography.采用人源钠碘转运体为基础的正电子发射断层扫描技术对 HER2 卵巢癌进行靶向 CAR-NK 细胞成像。
Eur J Nucl Med Mol Imaging. 2024 Sep;51(11):3176-3190. doi: 10.1007/s00259-024-06722-w. Epub 2024 May 9.
6
Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types.工程水交换是一种用于多种细胞类型磁共振成像的安全有效方法。
J Biol Eng. 2024 Apr 22;18(1):30. doi: 10.1186/s13036-024-00424-5.
7
A dual-gene reporter-amplifier architecture for enhancing the sensitivity of molecular MRI by water exchange.一种用于通过水交换提高分子磁共振成像灵敏度的双基因报告-放大结构。
bioRxiv. 2024 Jan 25:2024.01.22.576672. doi: 10.1101/2024.01.22.576672.
8
PSMA imaging as a non-invasive tool to monitor inducible gene expression in vivo.前列腺特异性膜抗原（PSMA）成像作为一种监测体内诱导基因表达的非侵入性工具。
EJNMMI Res. 2024 Jan 4;14(1):3. doi: 10.1186/s13550-023-01063-5.
9
Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types.工程水交换是一种用于多种细胞类型磁共振成像的安全有效方法。
bioRxiv. 2023 Nov 10:2023.11.07.566095. doi: 10.1101/2023.11.07.566095.
10
PET reporter systems for the brain.用于大脑的 PET 报告系统。
Trends Neurosci. 2023 Nov;46(11):941-952. doi: 10.1016/j.tins.2023.08.007. Epub 2023 Sep 19.
The art of reporter proteins in science: past, present and future applications.
报告蛋白在科学中的艺术：过去、现在和未来的应用。
BMB Rep. 2010 Jul;43(7):451-60. doi: 10.5483/bmbrep.2010.43.7.451.
4
Development of a universal anti-polyethylene glycol reporter gene for noninvasive imaging of PEGylated probes.开发一种通用的抗聚乙二醇报告基因，用于非侵入性成像聚乙二醇化探针。
J Nucl Med. 2010 Jun;51(6):933-41. doi: 10.2967/jnumed.109.071977. Epub 2010 May 19.
5
Challenges in the translation of cardiovascular cell therapy.心血管细胞治疗的翻译挑战。
J Nucl Med. 2010 May 1;51 Suppl 1(Suppl 1):122S-127S. doi: 10.2967/jnumed.109.068304. Epub 2010 Apr 15.
6
Indirect imaging of cardiac-specific transgene expression using a bidirectional two-step transcriptional amplification strategy.利用双向两步转录扩增策略间接成像心脏特异性转基因表达。
Gene Ther. 2010 Jul;17(7):827-38. doi: 10.1038/gt.2010.30. Epub 2010 Mar 18.
7
Two decades of clinical gene therapy--success is finally mounting.二十年的临床基因治疗——成功终于日益显现。
Discov Med. 2010 Feb;9(45):105-11.
8
A transposon and transposase system for human application.用于人类应用的转座子和转座酶系统。
Mol Ther. 2010 Apr;18(4):674-83. doi: 10.1038/mt.2010.2. Epub 2010 Jan 26.
9
Dendritic cell recovery post-lymphodepletion: a potential mechanism for anti-cancer adoptive T cell therapy and vaccination.树突状细胞在淋巴耗竭后的恢复：一种用于抗癌过继性 T 细胞治疗和疫苗接种的潜在机制。
Cancer Immunol Immunother. 2010 Mar;59(3):341-53. doi: 10.1007/s00262-009-0792-6. Epub 2009 Nov 18.
10
Radiolabelled oligonucleotides for imaging of gene expression with PET.放射性标记寡核苷酸用于正电子发射断层显像基因表达成像。
Curr Med Chem. 2009;16(33):4445-61. doi: 10.2174/092986709789712844.