Bonne C, Junquero D, Driot J Y, Coquelet C, Modat G
Laboratoire de Physiologie Cellulaire, Université de Montpellier I.
Ophtalmologie. 1990 Jan-Feb;4(1):84-7.
Corneal endothelium is a target tissue for retinoids and epidermal growth factor (EGF). We report here that retinoic acid and its synthetic analog, CBS-211 A (10(-8)-10(-7) M), enhance the mitogenic effect of EGF on cultured bovine corneal endothelial cells (BCEC). Furthermore, retinoid treatment increases the EGF-binding capacity of BCEC. The addition of cycloheximide (0.5 micrograms/ml) to cultures simultaneously with retinoids suppressed the retinoid effect on EGF-binding. These results suggest that retinoids could induce the expression of EGF receptors on BCEC as an early event. This study demonstrates a cooperation between retinoids and EGF in corneal endothelium and suggests that retinoids could have a beneficial effect on this tissue repair in vivo.
角膜内皮是类视黄醇和表皮生长因子（EGF）的靶组织。我们在此报告，视黄酸及其合成类似物CBS - 211 A（10^(-8)-10^(-7) M）可增强EGF对培养的牛角膜内皮细胞（BCEC）的促有丝分裂作用。此外，类视黄醇处理可增加BCEC的EGF结合能力。与类视黄醇同时向培养物中添加环己酰亚胺（0.5微克/毫升）可抑制类视黄醇对EGF结合的作用。这些结果表明，类视黄醇可作为早期事件诱导BCEC上EGF受体的表达。本研究证明了类视黄醇与EGF在角膜内皮中的协同作用，并表明类视黄醇可能对该组织的体内修复具有有益作用。
