Federal University of Rio de Janeiro, Avenida Carlos Chagas Filho, 393 RJ 21941-590, Brazil.
J Agric Food Chem. 2012 May 16;60(19):4826-32. doi: 10.1021/jf300602w. Epub 2012 May 3.
Consumption of plant food rich meals, such as feijoada, a traditional meal in Brazil, is associated with the reduction of chronic disease. The objectives of this study were to determine phytochemical content and antioxidant activity by chemical and cellular antioxidant assays (CAA) of feijoada with or without in vitro digestion. Feijoada showed no difference in phenolics and flavonoids after digestion. Bound and residue contributions to total phenolics were 20.9% and 32.2%, respectively, suggesting that phenolics reach the colon after intake. Flavonoids in residue and bound fractions represented 50% of total flavonoids. Antioxidant activity of feijoada without digestion was higher than that with digestion; however, it showed lower antiproliferative activity and CAA. Feijoada with in vitro digestion also yielded phenolics with higher CAA. Analyses of whole meals should be used to evaluate phytochemicals present in food mixtures consumed, especially with digestion models coupled with CAA resulting in information similar to those in physiological conditions.
食用富含植物性食物的膳食，如巴西的传统菜肴“黑豆饭”，与慢性病的减少有关。本研究的目的是通过化学和细胞抗氧化测定法（CAA）来确定黑豆饭在有或没有体外消化的情况下的植物化学物质含量和抗氧化活性。黑豆饭在消化后在酚类和类黄酮方面没有差异。结合和残留对总酚的贡献分别为 20.9%和 32.2%，这表明酚类物质在摄入后到达结肠。残渣和结合部分的类黄酮分别代表总类黄酮的 50%。未经消化的黑豆饭的抗氧化活性高于消化后的抗氧化活性，但它显示出较低的增殖抑制活性和 CAA。进行体外消化的黑豆饭也产生了具有更高 CAA 的酚类物质。应该使用完整膳食的分析来评估混合食物中存在的植物化学物质，特别是使用与 CAA 相结合的体外消化模型，从而获得类似于生理条件下的信息。
