Joshi Subhash C, Mathela Chandra S
Department of Chemistry, Kumaun University, Nainital, Uttarakhand, India.
Pharmacognosy Res. 2012 Apr;4(2):80-4. doi: 10.4103/0974-8490.94721.
Lindera pulcherrima (Nees.) Benth. ex Hook. f. (Family: Lauraceae), an evergreen shrub, is an important medicinal plant distributed in temperate Himalayan regions. The leaves and bark are used as spice in cold, fever, and cough.
In this study, the terpenoid composition, antioxidant, and antibacterial activities of the leaf essential oil and its major constituents are being analyzed.
The in vitro antioxidant activity showed a potent free radical scavenging activity for the essential oil as evidenced by a low IC(50) value for DPPH radical followed by furanodienone (0.087 ± 0.03 and 1.164 ± 0.58 mg/ml respectively) and the inhibition of lipid peroxidation for the oil and furanodienone also followed the same order (IC(50) 0.74 ± 0.13 and 2.12 ± 0.49 mg/ml, respectively). The oil and the constituents were also tested against three Gram negative (Escherichia coli, Salmonella enterica enterica, and (Pasturella multocida) and one Gram positive (Staphylococcus aureus) bacteria. The essential oil was effective against S. aureus (IZ = 19.0 ± 0.34; MIC 3.90 μl/ml) while furanodienone showed potent activity against E. coli and S. enterica enterica (IZ = 18.0 ± 0.14 and 16.0 ± 0.10 respectively). On the other hand, curzerenone was found to be slightly effective against E. coli (IZ = 10.8 ± 0.52). The MIC value of the essential oil was least against S. aureus (MIC = 3.90 μl/ml) and that of furanodienone against E. coli (MIC = 3.90 μl/ml).
山胡椒（樟科）是一种常绿灌木，是分布于喜马拉雅温带地区的重要药用植物。其叶和树皮被用作治疗感冒、发烧和咳嗽的香料。
在本研究中，对叶精油及其主要成分的萜类成分、抗氧化和抗菌活性进行了分析。
体外抗氧化活性表明，该精油具有较强的自由基清除活性，DPPH自由基的IC（50）值较低，其次是呋喃二烯酮（分别为0.087±0.03和1.164±0.58毫克/毫升），该精油和呋喃二烯酮对脂质过氧化的抑制作用也遵循相同顺序（IC（50）分别为0.74±0.13和2.12±0.49毫克/毫升）。该精油及其成分还针对三种革兰氏阴性菌（大肠杆菌、肠炎沙门氏菌和多杀性巴氏杆菌）和一种革兰氏阳性菌（金黄色葡萄球菌）进行了测试。该精油对金黄色葡萄球菌有效（抑菌圈直径IZ = 19.0±0.34；最低抑菌浓度MIC为3.90微升/毫升），而呋喃二烯酮对大肠杆菌和肠炎沙门氏菌显示出较强活性（IZ分别为18.0±0.14和16.0±0.10）。另一方面，莪术烯酮对大肠杆菌的效果稍差（IZ = 10.8±0.52）。该精油的最低抑菌浓度对金黄色葡萄球菌最低（MIC = 3.90微升/毫升），呋喃二烯酮对大肠杆菌的最低抑菌浓度（MIC = 3.90微升/毫升）。
