Apáti P, Szentmihályi K, Kristó Sz T, Papp I, Vinkler P, Szoke E, Kéry A
Faculty of Pharmacy, Department of Pharmacognosy, Semmelweis University, Budapest Ulloi str.26, Hungary.
J Pharm Biomed Anal. 2003 Aug 8;32(4-5):1045-53. doi: 10.1016/s0731-7085(03)00207-3.
In this study the correlation of phytochemical characteristics and antioxidative properties of classical herbal tea extracts-Infusum solidaginis, Decoctum solidaginis, Maceratum solidaginis-and tinctures prepared by various concentration of ethanol (40, 70, 96% v/v) have been examined for the release of flavonoids and their antioxidant activity. Quantitative and composition determination of flavonoids were carried out by spectrophotometry, high-performance liquid chromatography and capillary electrophoresis, respectively. Hydrogen-donating ability and reducing power properties were used to define in vitro radical scavenging activity of Solidago extracts, but integral antioxidative capacity was determined by luminometry (Photochem), calculating the ascorbic acid equivalents. Chlorogenic acid, quercetin-3-O-beta-D-rutinoside, quercetin-3-O-beta-D-galactoside, quercetin-3-O-beta-D-glucoside, quercetin-3-O-beta-D-rhamnoside, kaempferol-3-O-alpha-L-rhamnoside and quercetin were confirmed by retention times and UV spectra. Based on the dissolution rate, variance of flavonoid release and ascorbic acid equivalents it was concluded, that Tinctura solidaginis (70% v/v ethanol) and Infusum solidaginis are the most appropriate preparations.
在本研究中，考察了经典药草茶提取物（一枝黄花浸剂、一枝黄花煎剂、一枝黄花浸渍剂）以及用不同浓度乙醇（40%、70%、96% v/v）制备的酊剂的植物化学特征与抗氧化性能之间的相关性，以研究黄酮类化合物的释放及其抗氧化活性。分别采用分光光度法、高效液相色谱法和毛细管电泳法对黄酮类化合物进行定量和成分测定。通过供氢能力和还原能力来定义一枝黄花提取物的体外自由基清除活性，但通过发光分析法（Photochem）计算抗坏血酸当量来测定整体抗氧化能力。通过保留时间和紫外光谱确认了绿原酸、槲皮素 - 3 - O - β - D - 芸香糖苷、槲皮素 - 3 - O - β - D - 半乳糖苷、槲皮素 - 3 - O - β - D - 葡萄糖苷、槲皮素 - 3 - O - β - D - 鼠李糖苷、山柰酚 - 3 - O - α - L - 鼠李糖苷和槲皮素。基于溶解速率、黄酮类化合物释放的差异和抗坏血酸当量得出结论，一枝黄花酊剂（70% v/v乙醇）和一枝黄花浸剂是最合适的制剂。
