Dahlbäck M, Karlberg I B, Prytz M, Jarby R
AB Draco, Pharmacological laboratory, Lund, Sweden.
Allergy. 1990 Oct;45(7):487-96. doi: 10.1111/j.1398-9995.1990.tb00524.x.
We wanted to study the effect of anti-asthma drugs on antigen-induced dyspnea in conscious Sprague Dawley (S.D.) rats. A line of dyspnea bred rats was produced, where all immunized animals respond with dyspnea when challenged with aerosolized ovalbumin (OA). The animals were immunized intraperitoneally (i.p.) with OA (10 micrograms) together with Al(OH)3 (100 mg) and challenged 2 to 3 weeks later with OA aerosol. We examined the effects of terbutaline (TERB), disodium cromoglycate (DSCG), atropine (ATRO), theophylline (THEO), a 5-HT receptor antagonist methysergide (METH), and two glucocorticosteroids (GCS) budesonide (BUD) and dexamethasone (DEX), on this response. The drugs were given locally either by intratracheal (i.t.) instillation or by aerosol. The rats were placed one by one in an air tight box and the breathing pattern was recorded. Parameters such as time of onset of dyspnea after end of challenge (elapsed time), duration and occurrence of dyspnea, were used to quantify the response. In the vehicle- treated group nearly all animals responded to OA with signs of dyspnea. TERB was the only drug effective when given as an aerosol, whereas when given i.t., also DEX and METH inhibited the dyspnea. Dyspnea is a serious response and can only be alleviated by the most effective anti-asthma drugs.
我们想要研究抗哮喘药物对清醒的斯普拉格-道利（S.D.）大鼠抗原诱导的呼吸困难的影响。培育出了一组呼吸困难的大鼠品系，所有免疫后的动物在受到雾化卵清蛋白（OA）攻击时都会出现呼吸困难。这些动物腹腔内注射（i.p.）10微克OA和100毫克氢氧化铝（Al(OH)₃）进行免疫，2至3周后用OA气雾剂进行攻击。我们研究了特布他林（TERB）、色甘酸钠（DSCG）、阿托品（ATRO）、茶碱（THEO）、5-羟色胺受体拮抗剂麦角新碱（METH）以及两种糖皮质激素（GCS）布地奈德（BUD）和地塞米松（DEX）对这种反应的影响。药物通过气管内（i.t.）滴注或气雾剂进行局部给药。将大鼠逐个放入气密箱中并记录呼吸模式。使用诸如攻击结束后呼吸困难开始的时间（经过时间）、呼吸困难的持续时间和发生率等参数来量化反应。在赋形剂处理组中，几乎所有动物对OA都有呼吸困难的迹象。TERB是唯一作为气雾剂给药时有效的药物，而当气管内给药时，DEX和METH也能抑制呼吸困难。呼吸困难是一种严重的反应，只有最有效的抗哮喘药物才能缓解。
