Department of Materials Science and Engineering, Fujian University of Technology, Fuzhou 350108, China.
Sci China Life Sci. 2012 Mar;55(3):236-40. doi: 10.1007/s11427-012-4291-1. Epub 2012 Apr 14.
Biomimetic scaffolds are appealing products for the repair of bone defects using tissue engineering strategies. In the present study, novel biomimetic composite scaffolds, with similar properties to natural bone, were prepared, blended and cross-linked with bioactive glass, type I collagen and phosphatidylserine. When exposed to cell culture solution in the absence of a cellular source, the composite scaffolds form crystals with octahedral structure. These crystals are similar to the products derived from MC3T3-E1 cell mineralization within the composite scaffolds, with respect to both composition and morphology. Furthermore, crystals with octahedral structure were observed to develop into plate-like hydroxyapatite. The bio-mineralization behavior of the composite scaffolds is likely influenced by inorganic components. Finally, a rabbit tibia defect model shows that the highly bioactive properties of the investigated composites result in excellent bone repair.
仿生支架是一种很有吸引力的产品，可用于组织工程策略修复骨缺损。在本研究中，新型仿生复合支架，具有类似于天然骨的特性，用生物活性玻璃、I 型胶原和磷脂酰丝氨酸混合和交联而成。当暴露于无细胞来源的细胞培养液中时，复合支架形成具有八面体结构的晶体。这些晶体在组成和形态上与复合支架内 MC3T3-E1 细胞矿化的产物相似。此外，观察到具有八面体结构的晶体发育成板状羟基磷灰石。复合支架的生物矿化行为可能受无机成分的影响。最后，兔胫骨缺损模型表明，所研究的复合材料具有的高生物活性特性可实现优异的骨修复。
