Department of Drug Chemistry and Technologies, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy.
J Mater Sci Mater Med. 2012 Jul;23(7):1715-22. doi: 10.1007/s10856-012-4644-0. Epub 2012 Apr 24.
In the present study, mechanical and protein delivery properties of a system based on the interpenetration of calcium-alginate (Ca-Alg) and dextran-methacrylate (Dex-MA) networks are shown. Interpenetrated hydrogels beads were prepared by means of the alginate chains crosslinking with calcium ions, followed by the exposure to UV light that allows the Dex-MA network formation. Optical microscope analysis showed an average diameter of the IPN beads (Ca-Alg/Dex-MA) of 2 mm. This dimension was smaller than that of Ca-Alg beads because of the Dex-MA presence. Moreover, the strength of the IPN beads, and of their corresponding hydrogels, was influenced by the Dex-MA concentration and the crosslinking time. Model proteins (BSA and HRP) were successfully entrapped into the beads and released at a controlled rate, modulated by changing the Dex-MA concentration. The enzymatic activity of HRP released from the beads was maintained. These novel IPN beads have great potential as protein delivery system.
在本研究中，展示了基于钙-海藻酸钠 (Ca-Alg) 和葡聚糖甲基丙烯酰胺 (Dex-MA) 网络互穿的系统的机械和蛋白质输送性能。通过用钙离子交联海藻酸盐链来制备互穿水凝胶珠，然后暴露于允许 Dex-MA 网络形成的紫外线下。光学显微镜分析显示，IPN 珠（Ca-Alg/Dex-MA）的平均直径为 2mm。由于 Dex-MA 的存在，该尺寸小于 Ca-Alg 珠的尺寸。此外，IPN 珠及其相应水凝胶的强度受 Dex-MA 浓度和交联时间的影响。模型蛋白（BSA 和 HRP）成功地包埋在珠中，并通过改变 Dex-MA 浓度以控制速率释放。从珠中释放的 HRP 的酶活性得以保持。这些新型的 IPN 珠具有作为蛋白质输送系统的巨大潜力。
