通过纳米通道中的横向电流对DNA进行表征。

DNA characterization by transverse electrical current in a nanochannel.

作者信息

Di Ventra Massimiliano, Krems Matt, Wilson James, Pershin Yuriy V

机构信息

Department of Physics, University of California, San Diego, CA, USA.

出版信息

Methods Mol Biol. 2012;870:149-63. doi: 10.1007/978-1-61779-773-6_9.

DOI:10.1007/978-1-61779-773-6_9

PMID:22528263

Abstract

We review an approach for the characterization of single-stranded DNA based on the statistical identification of single bases via transverse electronic transport while DNA translocates in a nanopore or nanochannel. We describe the theoretical methods used to demonstrate this method for experimentally realizable systems and discuss the different physical processes involved. Recent experimental reports have shown the validity of this approach, although further work is necessary to make this a practical fast sequencing tool.

摘要

我们回顾了一种基于单碱基统计识别的单链DNA表征方法，该方法通过在纳米孔或纳米通道中DNA易位时的横向电子传输来实现。我们描述了用于证明该方法在实验可实现系统中的理论方法，并讨论了其中涉及的不同物理过程。最近的实验报告已经证明了该方法的有效性，尽管要使其成为一种实用的快速测序工具还需要进一步的工作。